Diversity of the biosynthesis pathway for threonylcarbamoyladenosine (t(6)A), a universal modification of tRNA.
Diversity of the biosynthesis pathway for threonylcarbamoyladenosine (t(6)A), a universal modification of tRNA.
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DOI：
10.4161/15476286.2014.992277
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发表时间：
2014
期刊：
影响因子：
4.1
通讯作者：
de Crécy-Lagard V
中科院分区：
文献类型：
--
作者：
Thiaville PC;Iwata-Reuyl D;de Crécy-Lagard V
tRNA 修饰领域拥有丰富的文献，涉及 40 多年前的生化分析，但许多相应的基因直到最近十年才被发现。近年来，比较基因组驱动的分析已经能够鉴定基因并随后表征负责 N6-苏氨甲酰基腺苷 (t6A) 的酶。这种通用修饰位于反密码子茎环中与 tRNA 反密码子相邻的第 37 位，几乎存在于所有解码 ANN 密码子的 tRNA 中。 t6A 生物合成酶和合成途径现已确定，揭示了一组核心酶和王国特异性变异。本综述重点阐述了途径、合成基因的多样性，并提出了 t6A 合成酶的新命名法。
The tRNA modification field has a rich literature covering biochemical analysis going back more than 40 years, but many of the corresponding genes were only identified in the last decade. In recent years, comparative genomic-driven analysis has allowed for the identification of the genes and subsequent characterization of the enzymes responsible for N6-threonylcarbamoyladenosine (t6A). This universal modification, located in the anticodon stem-loop at position 37 adjacent to the anticodon of tRNAs, is found in nearly all tRNAs that decode ANN codons. The t6A biosynthesis enzymes and synthesis pathways have now been identified, revealing both a core set of enzymes and kingdom-specific variations. This review focuses on the elucidation of the pathway, diversity of the synthesis genes, and proposes a new nomenclature for t6A synthesis enzymes.