GASTRIC INTERLEUKIN-8 AND IGA IL-8 AUTOANTIBODIES IN HELICOBACTER-PYLORI INFECTION
GASTRIC INTERLEUKIN-8 AND IGA IL-8 AUTOANTIBODIES IN HELICOBACTER-PYLORI INFECTION
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DOI：
10.1111/j.1365-3083.1993.tb01666.x
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发表时间：
1993-01-01
影响因子：
3.7
通讯作者：
LINDLEY, IJD
中科院分区：
文献类型：
--
作者：
CRABTREE, JE;PEICHL, P;LINDLEY, IJD
幽门螺杆菌引起的胃感染通常以中性粒细胞浸润为特征。在短期体外培养过程中，通过人胃窦活检检查了中性粒细胞激活肽 (NAP-1/IL-8) 和针对 IL-8 的粘膜 IgA 自身抗体的产生。 84% 胃窦粘膜正常的幽门螺杆菌阴性患者分泌可检测的 IL-8（范围 < 0.07-61.5 ng/mg 活检蛋白，n = 19）。 4 名反应性胃炎患者和 10 名非活动性胃炎患者的浓度与正常粘膜受试者没有显着差异。在患有活动性胃炎和中性粒细胞浸润上皮的幽门螺杆菌阳性患者中 (n = 17)，相对于正常粘膜受试者 (P < 0.0001)、非活动性胃炎 (P < 0.001) 和反应性胃炎 (P < 0.01)，IL-8 分泌显着增加。活动性胃炎中IL-8浓度与上皮表面变性程度显着相关（r = 0.64）。 19 名患者（13 名活动性胃炎，4 名非活动性胃炎）存在 IgA 自身抗体，其浓度与 IL-8 产生显着相关（P < 0.001）。 IL-8的胃合成可能是调节幽门螺杆菌感染患者粘膜中性粒细胞浸润和活化的重要因素。针对 IL-8 的 IgA 抗体的局部产生可能代表宿主的下调反应，以限制与慢性细菌感染相关的粘膜损伤。
Gastric infection with Helicobacter pylori is frequently characterized by neutrophil infiltration. The production of the neutrophil-activating peptide (NAP-1/IL-8) and mucosal IgA autoantibodies to IL-8 by human antral biopsies have been examined during short-term in vitro culture. Detectable IL-8 was secreted by 84% of H. pylori-negative patients with normal antral mucosa (range < 0.07-61.5 ng/mg biopsy protein, n = 19). Concentrations in 4 patients with reactive gastritis and 10 with inactive gastritis were not significantly different from subjects with normal mucosa. In H. pylori-positive patients with active gastritis and neutrophil infiltration into the epithelium (n = 17) IL-8 secretion was significantly increased relative to subjects with normal mucosa (P < 0.0001), inactive gastritis (P < 0.001) and reactive gastritis (P < 0.01). IL-8 concentrations in active gastritis were significantly correlated with the extent of epithelial surface degeneration (r = 0.64). IgA autoantibodies were present in 19 patients (13 active, 4 inactive gastritis) and concentrations were significantly correlated with IL-8 production (P < 0.001). Gastric synthesis of IL-8 is likely to be an important factor in regulating mucosal neutrophil infiltration and activation in patients with H. pylori infection. The local production of IgA antibodies to IL-8 may represent a down-regulatory response of the host to limit mucosal damage associated with a chronic bacterial infection.