Paraoxonase-1 activity is related to Trichinella spirals-induced hepatitis in rats
Paraoxonase-1 activity is related to Trichinella spirals-induced hepatitis in rats
复制标题
Paraoxonase-1 活性与旋毛虫引起的大鼠肝炎有关
DOI：
10.1111/eci.12731
复制
发表时间：
2017
影响因子：
5.5
通讯作者：
Nariaki Nonaka and Yoichiro Horii
中科院分区：
文献类型：
--
作者：
Ayman Samir Farid;Eman Mohamed Fath;Shogo Mido;Nariaki Nonaka and Yoichiro Horii
背景人们对慢性人畜共患线虫旋毛虫感染对肝脏炎症的潜在不利影响及其与对氧磷酶 (PON)-1 和丁酰胆碱酯酶 (BuChE) 活性的关系知之甚少。因此，我们旨在检查 T 的影响。 螺旋体感染对肝脏PON1合成的影响。方法用2500只螺旋体一期幼虫(L1)感染Wistar大鼠。 螺旋藻，并评估血清 PON1 和 BuChE 活性。感染后 9 周内评估 PON1、BuChE 以及各种细胞因子和趋化因子 [白细胞介素 (IL)-1、IL-4、IL-6、IL-10、肿瘤坏死因子 (TNF)-α、单核细胞趋化蛋白 (MCP)-1、巨噬细胞炎症蛋白 (MIP)-1α 和转化生长因子 (TGF)-β1] 的肝脏表达水平（p.i.）。还研究了这些变化对肝细胞凋亡程度的影响。结果从注射后第 2 天到第 7 周，大鼠旋毛虫感染导致血清 PON1 活性显着降低。和 BuChE 活动（从PI 的第 4 天到 2 周）此外，在寄生虫生命周期的肠内阶段，螺旋毛虫感染增加了血清促炎细胞因子 IL-1、IL-6 和 TNF-α 以及趋化因子 MCP-1、MIP-1α 和 TGF-β1。抗炎细胞因子IL-4和IL-10在前者的肠内期和后者的肌肉期显示出显着增加。这些与肝脏炎症和细胞凋亡有关。这些事件通常会降低肝脏 PON1 和 BuChE mRNA 表达。结论 针对 T. 大鼠的螺旋体感染与肝脏炎症以及随后血清 PON1 和 BuChE 活性的降低有关。
BackgroundLittle is known about the potential adverse effects of a chronic zoonotic nematodeTrichinella spiralisinfection on hepatic inflammation and its relationship to paraoxonase (PON)‐1 and butyrylcholinesterase (BuChE) activities. Therefore, we aimed to examine the effects ofT. spiralisinfection on hepatic synthesis of PON1.MethodsWistar rats were infected with 2500 first‐stage larvae (L1) ofT. spiralis, and serum PON1 and BuChE activities were evaluated. Hepatic expression levels of PON1, BuChE and various cytokines and chemokines [interleukin (IL)‐1, IL‐4, IL‐6, IL‐10, tumour necrosis factor (TNF)‐α, monocyte chemoattractant protein (MCP)‐1, macrophage inflammatory protein (MIP)‐1α, and transforming growth factor (TGF)‐β1] were evaluated for up to 9 weeks post‐infection (p.i.). The effect of these changes on the degree of hepatic apoptosis was also investigated.ResultsTrichinella spiralisinfection in rats induced significant decreases in serum PON1 activities from day 2 until week 7 p.i. and BuChE activity starting from day 4 until 2 weeks p.i. Moreover,T.spiralisinfection increased serum pro‐inflammatory cytokines IL‐1, IL‐6 and TNF‐α as well as chemokines MCP‐1, MIP‐1α and TGF‐β1 during the enteral phase of the parasite life cycle. The anti‐inflammatory cytokines IL‐4 and IL‐10 showed significant increases during the enteral phase for the former and the muscle phase for the latter. These were associated with hepatic inflammation and apoptosis. These events typically decreased hepatic PON1 and BuChE mRNA expression.ConclusionsImmune responses mounted againstT. spiralisinfection in rats were associated with hepatic inflammation and a subsequent decrease in serum PON1 and BuChE activities.