URINARY-EXCRETION HALF-LIFE OF DELTA-1-TETRAHYDROCANNABINOL-7-OIC ACID IN HEAVY MARIJUANA USERS AFTER SMOKING
URINARY-EXCRETION HALF-LIFE OF DELTA-1-TETRAHYDROCANNABINOL-7-OIC ACID IN HEAVY MARIJUANA USERS AFTER SMOKING
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DOI：
10.1093/jat/13.4.218
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发表时间：
1989-07-01
影响因子：
2.5
通讯作者：
HALLDIN, MM
中科院分区：
文献类型：
--
作者：
JOHANSSON, E;HALLDIN, MM
尿中总排泄量。delta。1-四氢大麻酚（. delta .1- thc）代谢物，特别强调。delta。1-四氢大麻酚-7-酸1- thc -7-酸)，对13名重度大麻使用者在服用。delta后进行了研究。1-THC，然后是四周的停药期。. delta的总金额。1-四氢大麻酚代谢物和。delta水平。1- thc -7-酸可在戒断后随访25 d，采用EMIT d.a.u.大麻素测定和高效液相色谱法（HPLC）。尿排泄的半衰期，由。delta的浓度计算。1- thc -7-酸与时间的关系，范围为0.8-9.8天，平均（±）。SD) 3.0±±。2.3天。大部分。delta。1- thc -7-酸作为偶联物排出体外，只有微量的未偶联的。delta。检测1-THC-7-oic酸。. delta的总浓度。将尿中的1- thc -7-酸浓度与半定量EMIT d.a.u.大麻素测定法获得的“交叉反应大麻素”浓度在20-75 ng/ML的线性范围内进行比较。“EMIT浓度”的平均比值/. delta。HPLC分析得到的1- thc -7-酸浓度为1.23 +-。78份尿样84% （C.V.）。高效液相色谱法（HPLC）证实83%的样品EMIT呈阳性（截止值为20 ng/mL）。
The urinary excretion of the total amount of .DELTA.1-tetrahydrocannabinol (.DELTA.1-THC) metabolites, with special emphasis on .DELTA.1-tetrahydrocannabinol-7-oic acid (.DELTA.1-THC-7-oic acid), was studied in thirteen heavy Cannabis users after smoking administration of .DELTA.1-THC, followed by a four week discontinuation period. The total amount of .DELTA.1-THC metabolites and the levels of .DELTA.1-THC-7-oic acid could be followed up to 25 days after abstinence using EMIT d.a.u. cannabinoid assay and high-performance liquid chromatography (HPLC). The urinary excretion half-life, calculated from the concentrations of .DELTA.1-THC-7-oic acid versus time, ranged from 0.8-9.8 days with a mean (.+-. SD) of 3.0 .+-. 2.3 days. Most of the .DELTA.1-THC-7-oic acid was excreted as conjugate and only trace amounts of unconjugated .DELTA.1-THC-7-oic acid were detected. The total concentrations of .DELTA.1-THC-7-oic acid in urine were compared to the concentrations of "cross-reacting cannabinoids", within the linear range of 20-75 ng/ML, obtained in the semiquantitative EMIT d.a.u. cannabinoid assay. The average ratio of "EMIT concentrations"/.DELTA.1-THC-7-oic acid concentrations obtained by HPLC analysis was 1.23 .+-. 84% (C.V.) for 78 urine samples. A total of 83% of the samples with positive EMIT levels (cutoff 20 ng/mL) was confirmed by HPLC analysis (cutoff 7 ng/mL).