Exposure of phosphatidylserine is a general feature in the phagocytosis of apoptotic lymphocytes by macrophages
Exposure of phosphatidylserine is a general feature in the phagocytosis of apoptotic lymphocytes by macrophages
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DOI：
10.1038/sj.cdd.4400473
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发表时间：
1999-02-01
影响因子：
12.4
通讯作者：
Schlegel, RA
中科院分区：
文献类型：
--
作者：
Krahling, S;Callahan, MK;Schlegel, RA
虽然不同的巨噬细胞利用不同的细胞表面受体来识别凋亡淋巴细胞，但间接证据表明，出现在凋亡淋巴细胞表面的磷脂酰丝氨酸（PS）参与了所有类型巨噬细胞的特异性识别。为了直接验证这种可能性，我们使用一种特异性结合PS的蛋白膜联蛋白V在凋亡细胞表面掩盖这种磷脂，用膜联蛋白V对凋亡淋巴细胞进行预孵育，阻断小鼠腹腔巨噬细胞、小鼠J774细胞系巨噬细胞和小鼠骨髓巨噬细胞的吞噬作用。同样，膜联蛋白V能够抑制脂质对称红细胞的吞噬，这是PS暴露的另一个靶细胞。这些结果直接表明，所有类型的巨噬细胞都依赖于暴露在凋亡淋巴细胞表面的PS进行识别和吞噬。
Although different macrophages exploit different cell surface receptors to recognize apoptotic lymphocytes, indirect evidence suggested that the phosphatidylserine (PS) that appears on the surface of lymphocytes undergoing apoptosis participates in specific recognition by all types of macrophages. To test this possibility directly, annexin V, a protein that specifically binds to PS, was used to mask this phospholipid on the apoptotic cell surface, Preincubation of apoptotic lymphocytes with annexin V blocked phagocytosis by elicited mouse peritoneal macrophages, macrophages of the mouse J774 cell line and mouse bone marrow macrophages. Similarly, annexin V was able to inhibit phagocytosis of lipid-symmetric erythrocytes, another target cell upon which PS is exposed. Together these results demonstrate directly that macrophages of all types depend on the PS exposed on the surface of apoptotic lymphocytes for recognition and phagocytosis.