Screening mercury levels in fish with a selective fluorescent chemosensor
Screening mercury levels in fish with a selective fluorescent chemosensor
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DOI：
10.1021/ja0557987
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发表时间：
2005-11-23
影响因子：
15
通讯作者：
Chang, CJ
中科院分区：
文献类型：
--
作者：
Yoon, S;Albers, AE;Chang, CJ
社会对鱼类及其他饮食和环境来源的有毒汞在人体内蓄积的关注，促使人们开发新的工具和策略，用于在广泛的实验室和现场环境中进行汞检测。在这里，我们报告的合成，性能和应用的选择性和敏感的小分子化学传感器的荧光筛选鱼的汞水平。Mercuryfluor-1（MF 1）是一种水溶性荧光素基试剂，其对Hg 2+的选择性优于竞争性分析物，并且是迄今为止用于报告水溶液中这种重金属离子的最大开启荧光响应（增加>170倍）。将这种化学选择性Hg 2+探针与微波消解方案相结合，提供了一种简便的方法，用于测定汞浓度范围为0.1至8 ppm的鱼样品中的汞含量，该范围与美国环境保护署（U.S. EPA）关于食用鱼中汞的最大安全水平（0.55 ppm）的标准非常匹配。
Societal concerns over toxic mercury accumulation in humans from fish and other dietary and environmental sources provide motivation to develop new tools and tactics for mercury detection in a wide range of laboratory and field settings. Here we report the synthesis, properties, and application of a selective and sensitive small-molecule chemosensor for fluorescence screening of mercury levels in fish. Mercuryfluor-1 (MF1) is a water-soluble, fluorescein-based reagent that features excellent selectivity for Hg2+over competing analytes and the largest turn-on fluorescence response to date (>170-fold increase) for reporting this heavy metal ion in aqueous solution. Combining this chemoselective Hg2+probe with a microwave digestion protocol provides a facile method for assaying mercury levels in fish samples with mercury concentrations spanning 0.1 to 8 ppm, a range well matched with the United States Environmental Protection Agency (U.S. EPA) standard for the maximum safe level of mercury in edible fish (0.55 ppm).