Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples
Evaluation of a real-time PCR kit for detecting Escherichia coli O157 in bovine fecal samples
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DOI：
10.1128/aem.70.3.1855-1857.2004
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发表时间：
2004-03-01
影响因子：
4.4
通讯作者：
Laegreid, WW
中科院分区：
文献类型：
--
作者：
Bono, JL;Keen, JE;Laegreid, WW
评价了市售实时快速PCR检测大肠杆菌O 157的能力。对纯培养物的敏感性和特异性均为99%。该试验检测到1 CFU的E.大肠杆菌O 157：H7 g（-1）。
A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g(-1) in artificially inoculated bovine feces following enrichment.