Determination of free and bounded phenolic acids in the rhizomes and herb of Sanguisorba officinalis L.
Determination of free and bounded phenolic acids in the rhizomes and herb of Sanguisorba officinalis L.
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DOI：
10.1515/cipms-2015-0083
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发表时间：
2015-12-01
影响因子：
0.3
通讯作者：
Bogucka-Kocka, Anna
中科院分区：
文献类型：
--
作者：
Biernasiuk, Anna;Wozniak, Michal;Bogucka-Kocka, Anna
采用2D-TLC法对地榆根茎和草本组织中水解物中的游离酸和酚酸进行了分析。共鉴定出15种酚酸。我们的工作表明，所有供试部位都含有鞣花酸、原儿茶酸、龙胆酸、对羟基苯甲酸、丁香酸、香草酸和阿魏酸，没食子酸和原儿茶酸占主导地位，而咖啡酸、对香豆酸、丁香酸、香草酸和阿魏酸在草本植物组织中含量丰富。然而，α-和β-间苯二酸仅在它们与糖和醇结合后释放出来的根茎酚酸部分中检测到。此外，绿原酸只以游离态存在于草本植物组织中，而对羟基苯乙酸是从草本植物提取物中结合释放出来的。
An analysis of the fractions of free acids and phenolic acids liberated by way of hydrolysis in the rhizomes and herbaceous tissues of Sanguisorba officinalis L. was conducted through utilizing the 2D-TLC method. Fifteen phenolic acids were identified. Our work showed that ellagic, protocatechuic, gentisic, p-hydroxybenzoic, syringic, vannilic and ferulic acids were common in all tested fractions, and gallic and protocatechuic acids were dominant in fractions obtained from the rhizomes, while caffeic, p-coumaric, syringic, vannilic and ferulic acids were abundant in the herbaceous tissues. However, alpha and beta-resorcylic acids were detected only in rhizome phenolic acid fractions liberated from their conjunction with sugars and alcohols. Furthermore, chlorogenic acid was present only in a free form in the herbaceous tissues, while p-hydroxyphenylacetic acid was liberated from conjunction from a herbaceous extract.