A new method for fish leucocyte counting and partial differentiation by flow cytometry
A new method for fish leucocyte counting and partial differentiation by flow cytometry
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DOI：
10.1006/fsim.2002.0413
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发表时间：
2002-11-01
影响因子：
4.7
通讯作者：
Nakanishi, T
中科院分区：
文献类型：
--
作者：
Inoue, T;Moritomo, T;Nakanishi, T
本文提出了一种简单、快速的分析鱼血细胞的方法。鲤鱼血液用含1杯/毫升DioC(6)(3)的Hanks‘s溶液稀释200倍，Dioc(6)(3)是一种荧光、亲脂的染料。染色10min后，用流式细胞仪(FACS)检测血细胞。通过不同的FL-1(绿色荧光)、FSC(前向散射)和SSC(侧散射)特性来鉴定几种血细胞群。FL-1 v.SSC或FSC v.SSC染色血细胞的点图显示五种不同的细胞群：红细胞、血小板和淋巴细胞的混合物、单核细胞、中性粒细胞和嗜碱性粒细胞。流式细胞仪计数的每种血细胞数与显微镜计数的血细胞数基本一致。(C)2002年由爱思唯尔科学有限公司出版。
A simple and rapid method for analysis of fish blood cells is presented. Carp (Cyprinus carpio) blood was diluted 200 times with Hanks'solution containing 1 mug/ml of DiOC(6)(3) which is a fluorescent, lipophilic dye. After staining for 10 min, the blood cells were measured by a flow cytometer (FACS). Several blood cell populations were identified by different FL-1 (green fluorescence), FSC (forward scatter), and SSC (side scatter) properties. FL-1 v. SSC or FSC v. SSC dot-plot of stained blood cells displayed five separate cell populations: erythrocytes; a mixture of thrombocytes plus lymphocytes; monocytes; neutrophils; and basophils. The number of each type of blood cell counted by the FACS was in good agreement with those counted microscopically. (C) 2002 Published by Elsevier Science Ltd.