Alpha-particle-induced neoplastic transformation in synchronized hybrid cells of HeLa and human skin fibroblasts.
Alpha-particle-induced neoplastic transformation in synchronized hybrid cells of HeLa and human skin fibroblasts.
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HeLa 和人类皮肤成纤维细胞同步杂交细胞中α粒子诱导的肿瘤转化。
DOI：
10.1080/095530097143022
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发表时间：
1997
影响因子：
2.6
通讯作者：
Redpath,JL
中科院分区：
文献类型：
--
作者：
Bettega,D;Calzolari,P;Piazzolla,A;Tallone,L;Redpath,JL
通过细胞周期测定细胞在0.30和0.15Gy4.3 MeV(LET 101keV/mum)α粒子照射下的存活率和致癌转化率。通过有丝分裂采集进行同步化，照射时间为采集后2~10h，相当于G1期和早期S期。在0.30Gy时，转化频率最高(1.6±0.3)×10~(-4)转化子/存活者，出现在有丝分裂采集后4h，对应于G1期中期，约为异步化群体(0.7±0.1)×10~(-4)转化子/存活者的两倍。0.15Gy射线也出现了类似的图案，尽管没有那么明显。这一结果与C3H10T1/2受照0.30Gy时的结果相似，在G1期中期和非同步群体中分别发现(1.8，0.4)×10-4和(0.8，0.4)×10-4转化子/存活者。这两项对101keV/mum阿尔法粒子的研究结果表明，G1中期细胞可能比异步细胞敏感高达两倍。然而，这样的因素不太可能足以代表细胞周期的转化热点，这是假设的，以解释反向剂量率效应。
Survival and oncogenic transformation frequencies were determined through the cell cycle in hybrid cells (HeLa human skin fibroblasts), exposed to 0.30 and 0.15 Gy 4.3 MeV (LET 101 keV/ mu m) alpha -particles. The cells were synchronized by mitotic collection and irradiated at times ranging from 2 to 10 h after collection, corresponding to G1 and early S. At 0.30 Gy the highest value in the transformation frequency (1.6 0.3)*10 -4 transformants/survivor, occurred 4 h after mitotic collection, corresponding to mid-G1 and was about twice as high as that for the asynchronous population (0.7 0.1)*10 -4 transformants/survivor. A similar pattern was seen at 0.15 Gy albeit less marked. The results are similar to previous findings with C3H10T1/2 exposed to 0.30 Gy where (1.8 0.4)*10 -4 and (0.8 0.4)*10 -4 transformants/survivor were found in mid-G1 and in the asynchronous population respectively. The results of both these studies with 101 keV/ mu m alpha particles indicate that mid-G1 cells may be more sensitive than asynchronous cells by up to a factor of two. However, it is unlikely that such a factor is sufficient to represent the cell cycle 'hot spot' for transformation postulated to explain the inverse dose-rate effect.