The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region.
The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region.
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DOI：
10.1038/s41598-020-80258-5
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发表时间：
2021-01-12
影响因子：
4.6
通讯作者：
Nishikawa A
中科院分区：
文献类型：
--
作者：
Kozono T;Sato H;Okumura W;Jogano C;Tamura-Nakano M;Kawamura YI;Rohrer J;Tonozuka T;Nishikawa A
Jaw 1/LRMP是一种II型膜整合蛋白，定位于内质网（ER）和外核膜。我们以前报道过，Jaw 1的功能是通过其羧基末端区域维持KASH蛋白的核形状，羧基末端区域是寡聚状态下核骨架和细胞骨架复合物的连接体的组成部分。虽然一些KASH蛋白通过胞质区域的寡聚化起到稳定蛋白质-蛋白质相互作用的作用，但是Jaw 1的寡聚化如何被调节的问题还没有完全理解。因此，我们集中在三个不同的区域上的胞质面的Jaw 1：N-末端区域，卷曲螺旋结构域和茎区域，在寡聚化。免疫共沉淀试验表明，其卷曲螺旋结构域是寡聚化位点的候选者。此外，我们的数据表明，N-末端区域防止异常寡聚化的Jaw 1作为一个固有的无序区域（IDR）。重要的是，在B16 F10细胞中，N-末端区域缺失突变体的异位表达引起了有组织的平滑ER（OSER）的形成，结构如核臂和轮。此外，这种OSER干扰寡聚体和相互作用物如III型肌醇1，4，5-三磷酸受体（IP 3R 3）和SUN 2的定位。总之，Jaw 1的N-末端区域抑制OSER的形成，作为IDR以维持ER膜上相互作用物的稳态定位。
Jaw1/LRMP is a type II integral membrane protein that is localized at the endoplasmic reticulum (ER) and outer nuclear membrane. We previously reported that a function of Jaw1 is to maintain the nuclear shape as a KASH protein via its carboxyl terminal region, a component of linker of nucleoskeleton and cytoskeleton complex in the oligomeric state. Although the oligomerization of some KASH proteins via the cytosolic regions serves to stabilize protein-protein interactions, the issue of how the oligomerization of Jaw1 is regulated is not completely understood. Therefore, we focused on three distinct regions on the cytosolic face of Jaw1: the N-terminal region, the coiled-coil domain and the stem region, in terms of oligomerization. A co-immunoprecipitation assay showed that its coiled-coil domain is a candidate for the oligomerization site. Furthermore, our data indicated that the N-terminal region prevents the aberrant oligomerization of Jaw1 as an intrinsically disordered region (IDR). Importantly, the ectopic expression of an N-terminal region deleted mutant caused the formation of organized smooth ER (OSER), structures such as nuclear karmellae and whorls, in B16F10 cells. Furthermore, this OSER interfered with the localization of the oligomer and interactors such as the type III inositol 1,4,5-triphosphate receptor (IP3R3) and SUN2. In summary, the N-terminal region of Jaw1 inhibits the formation of OSER as an IDR to maintain the homeostatic localization of interactors on the ER membrane.
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DOI：
10.1074/jbc.m109.071910
发表时间：
2010-01-29
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Haque F;Mazzeo D;Patel JT;Smallwood DT;Ellis JA;Shanahan CM;Shackleton S
通讯作者：
Shackleton S
影响因子：
4
作者：
Demetriadou A;Morales-Sanfrutos J;Nearchou M;Baba O;Kyriacou K;Tate EW;Drousiotou A;Petrou PP
通讯作者：
Petrou PP
影响因子：
14.9
作者：
Ishida T;Kinoshita K
通讯作者：
Kinoshita K
影响因子：
30.8
作者：
Gros-Louis, Francois;Dupre, Nicolas;Rouleau, Guy A.
通讯作者：
Rouleau, Guy A.
DOI：
10.1007/978-1-61779-927-3_22
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Chemes, Lucia B;Alonso, Leonardo G;de Prat-Gay, Gonzalo
通讯作者：
de Prat-Gay, Gonzalo