Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta.
Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta.
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DOI：
10.1016/s0021-9258(18)48258-0
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发表时间：
1987-05
期刊：
影响因子：
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通讯作者：
R. Ignotz;T. Endo;J. Massagu
中科院分区：
文献类型：
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作者：
R. Ignotz;T. Endo;J. Massagu
人血小板源转化生长因子-β1可增加间质和上皮细胞中细胞外基质蛋白、纤维连接蛋白和I型胶原的积聚。为了确定这种作用的基础，我们检测了经转化生长因子-β1处理的NRK-49大鼠成纤维细胞和L6E9大鼠成肌细胞中与纤维连接蛋白和α2(I)前胶原相对应的mRNAs的水平。转化生长因子-β1使这两种蛋白的mRNAs水平增加了几倍。这两种信使核糖核酸的作用动力学是相似的。在加入转化生长因子-β1后2 h，细胞内纤维连接蛋白和α2(I)前胶原mRNAs的表达明显增加，并在数天内维持在最高水平。放线菌素D可抑制转化生长因子-β1诱导的纤维连接蛋白和α2(I)前胶原mRNA水平的升高，但放线菌素D不能抑制这一作用。结果表明，转化生长因子-β1至少部分地通过诱导纤维连接蛋白和I型胶原mRNAs水平的协同升高来控制细胞外基质的组成和丰度。
Human platelet-derived transforming growth factor-beta (TGF-beta 1) increases the accumulation of the extracellular matrix proteins, fibronectin and type I collagen, in mesenchymal and epithelial cells. To determine the basis for this effect, we have examined the levels of mRNAs corresponding to fibronectin and alpha 2(I) procollagen in NRK-49 rat fibroblasts and L6E9 rat myoblasts treated with TGF-beta 1. TGF-beta 1 increased severalfold the levels of mRNAs for both proteins. The kinetics of this effect were similar for both mRNA species. The increase in fibronectin and alpha 2(I) procollagen mRNAs was detectable 2 h after addition of TGF-beta 1 to the cells and their maximal levels remained constant for several days. Actinomycin D, but not cycloheximide, inhibited the increase in fibronectin and alpha 2(I) procollagen mRNA levels induced by TGF-beta 1. The results indicate that TGF-beta 1 controls the composition and abundance of extracellular matrices at least in part by inducing a coordinate increase in the levels of fibronectin and type I collagen mRNAs.