Temporal patterns of gene expression in murine cutaneous burn wound healing
Temporal patterns of gene expression in murine cutaneous burn wound healing
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DOI：
10.1152/physiolgenomics.00101.2003
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发表时间：
2004-02-13
影响因子：
4.6
通讯作者：
Mozingo, DW
中科院分区：
文献类型：
--
作者：
Feezor, RJ;Paddock, HN;Mozingo, DW
在二度烫伤后，损伤小鼠皮肤中基因表达的总体变化被表征。在65 ℃水中浸泡45 s后2 h、3天和14 d，从未受伤和烧伤小鼠中收获背部皮肤。使用AffyesterU 74 Av 2基因芯片调查基因表达，并使用层次聚类和监督分析分析基因表达模式。烧伤后许多基因的表达发生了显着变化，其中最大的变化出现在烧伤后3天和14天。使用错误发现率为1%或5%的监督分析，192或1,116个基因的表达差异分别区分了未烧伤的皮肤和烧伤后的三个时间点。基因表达主要是短暂的和时间依赖性的上调。192个识别基因中只有24个在烧伤后表达下调。在烧伤后的整个14天内，没有基因表现出持续的表达增加。基因本体显示，在急性时间间隔的炎症和蛋白酶基因的整合上调，并在损伤后3或14天的细胞骨架和肌肉收缩基因的减少。二度烧伤后，烧伤创面中基因表达的总体模式在数周内以时间依赖性方式发生显著变化，这些变化可以根据基因的生物学相关性进行分类。
The global changes in gene expression in injured murine skin were characterized following a second-degree scald burn. Dorsal skin was harvested from uninjured and from burned mice at 2 h and at 3 and 14 days following immersion in 65degreesC water for 45 s. Gene expression was surveyed using an Affymetrix U74Av2 GeneChip, and patterns of gene expression were analyzed using hierarchical clustering and supervised analysis. Burn injury produced significant alterations in the expression of a number of genes, with the greatest changes seen 3 and 14 days after the scald burn. Using a supervised analysis with a false discovery rate of 1% or 5%, differences in the expression of 192 or 1,116 genes, respectively, discriminated among the unburned skin and the three time points after the burn injury. Gene expression was primarily a transient and time-dependent upregulation. The expression of only 24 of the 192 discriminating genes was downregulated after the burn injury. No gene exhibited a sustained increase in expression over the entire 14 days following the burn injury. Gene ontologies revealed an integrated upregulation of inflammatory and protease genes at acute time intervals, and a diminution of cytoskeletal and muscle contractile genes at 3 or 14 days after the injury. Following a second-degree scald burn, global patterns of gene expression in the burn wound change dramatically over several weeks in a time-dependent manner, and these changes can be categorized based on the biological relevance of the genes.