Giant magnetoresistive biochip for DNA detection and HPV genotyping
Giant magnetoresistive biochip for DNA detection and HPV genotyping
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DOI：
10.1016/j.bios.2008.03.030
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发表时间：
2008-09-15
影响因子：
12.6
通讯作者：
Wang, Shan X.
中科院分区：
文献类型：
--
作者：
Xu, Liang;Yu, Heng;Wang, Shan X.
研制了一种基于自旋阀传感器阵列和磁性纳米颗粒标记的巨磁阻生物芯片，用于廉价、灵敏、可靠的DNA检测。本实验检测到的DNA靶点是人乳头状病毒（HPV）质粒扩增的PCR产物。在大多数情况下，PCR后的目标DNA浓度在10 nM左右，但也可以检测到10 pM的浓度，这在合成DNA样品的实验中得到了证明。探针寡核苷酸固定化采用了一种温和但具有高度特异性的表面化学方法。为了降低传感器中的1/f噪声，采用双调制技术进行信号检测。进行了12次测定，准确度约为90%。磁信号与扫描电镜（SEM）测量的颗粒覆盖数据一致。最近对微流体的研究表明，有可能将分析时间缩短到一小时以下。这是首次使用质粒衍生样品进行磁性DNA检测。这项研究为GMR传感器可用于生物医学应用提供了直接证据。(C) 2008 Elsevier B.V.版权所有
A giant magnetoresistive (GMR) biochip based on spin valve sensor array and magnetic nanoparticle labels was developed for inexpensive, sensitive and reliable DNA detection. The DNA targets detected in this experiment were PCR products amplified from Human Papillornavirus (HPV) plasmids. The concentrations of the target DNA after PCR were around 10 nM in most cases, but concentrations of 10 pM were also detectable, which is demonstrated by experiments with synthetic DNA samples. A mild but highly specific surface chemistry was used for probe oligonucleotide immobilization. Double modulation technique was used for signal detection in order to reduce the 1/f noise in the sensor. Twelve assays were performed with an accuracy of approximately 90%. Magnetic signals were consistent with particle coverage data measured with Scanning Electron Microscopy (SEM). More recent research on microfluidics showed the potential of reducing the assay time below one hour. This is the first demonstration of magnetic DNA detection using plasmid-derived samples. This study provides a direct proof that GMR sensors can be used for biomedical applications. (C) 2008 Elsevier B.V. All rights reserved.