Preventive effect of recombinant human lactoferrin in a rabbit preterm delivery model
Preventive effect of recombinant human lactoferrin in a rabbit preterm delivery model
复制标题
DOI：
10.1016/j.ajog.2005.01.013
复制
发表时间：
2005-04-01
影响因子：
9.8
通讯作者：
Kato, A
中科院分区：
文献类型：
--
作者：
Hasegawa, A;Otsuki, K;Kato, A
目的：乳铁蛋白是一种存在于子宫颈粘液和羊水中的铁结合糖蛋白，具有抵抗粘膜感染的作用。本研究探讨重组人乳铁蛋白对早产兔模型的影响。研究设计：将麻醉的兔随机分为两组，一组接种大肠杆菌，另一组在接种前2小时将重组人乳铁蛋白插入子宫颈（条件A：盐水+盐水;条件B：大肠杆菌+盐水;条件C：大肠杆菌+重组人乳铁蛋白）。使用宫腔镜和无菌聚乙烯套管将大肠杆菌、盐水溶液和重组人乳铁蛋白插入宫颈。监测接种后胎儿存活率和分娩天数，并测量母体血清和羊水中肿瘤坏死因子-α的浓度。条件A、B和C的胎儿存活率分别为95.7%、0%和32.6%，而妊娠持续时间分别为7.00 +/- 0天、3.25 +/- 0.43天、结论：宫颈注射重组人乳铁蛋白可增加胎儿存活率，延长妊娠时间。乳铁蛋白具有抗炎作用以及抗菌作用，表明重组人乳铁蛋白具有在临床环境中预防源自宫颈感染的早产的潜力。(c)2005年爱思唯尔公司All rights reserved.
Objective: Lactoferrin, an iron-binding glycoprotein found in cervical mucus and amniotic fluid, plays a defensive role against mucosal infections. This study examined the effect of recombinant human lactoferrin on preterm delivery in a rabbit model.Study design: Anesthetized rabbits were randomly assigned to receive either inoculation with Escherichia coli or saline solution and to receive treatment with or without recombinant human lactoferrin inserted into the cervix 2 hours before bacterial inoculation (condition A: saline + saline; condition B: E coli + saline; condition C: E coli + recombinant human lactoferrin). E coli, saline solution, and recombinant human lactoferrin were inserted into the cervix using a hysteroscope and a sterile polyethylene cannula. Fetus survival rate and days to delivery after inoculation were monitored and tumor necrosis factor-alpha concentrations were measured in maternal serum and amniotic fluid.Results: Fetus survival for conditions A, B, and C were 95.7%, 0%, and 32.6%, respectively, whereas pregnancy continuation was 7.00 +/- 0 days, 3.25 +/- 0.43 days, and 4.85 +/- 1.77 days, respectively.Conclusion: Cervical recombinant human lactoferrin administration increased fetal survival and extended pregnancy. Lactoferrin has an anti-inflammatory action as well as an antibacterial action, suggesting that recombinant human lactoferrin has the potential to prevent preterm delivery originating from cervical infection in the clinical setting. (c) 2005 Elsevier Inc. All rights reserved.