Expression and regulation of chemokine genes in the mouse uterus during pregnancy.
Expression and regulation of chemokine genes in the mouse uterus during pregnancy.
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妊娠期间小鼠子宫趋化因子基因的表达和调控。
DOI：
10.1006/cyto.1999.0513
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Kanakaraj,K
中科院分区：
文献类型：
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作者：
Wood,GW;Hausmann,EH;Kanakaraj,K
白细胞在交配后、着床期间和胎盘发育期间积聚在妊娠小鼠子宫中。白细胞数量的变化主要是由于从血液中募集，而不是局部增殖，但对潜在的募集机制知之甚少。交配诱导的粒细胞和巨噬细胞募集部分是由于精浆中存在的促炎因子和趋化因子。妊娠后期巨噬细胞的积聚似乎部分是由卵巢囊肿刺激的CSF-1产生引起的，部分是由其他尚未鉴定的子宫趋化因子引起的。目前的研究是为了评估妊娠期间子宫中趋化因子的产生。用北方印迹法检测子宫组织中NSI/KC（KC）、巨噬细胞趋化蛋白1（MCP 1）、巨噬细胞炎性蛋白1 α（MIP 1 α）和调节失活、正常T细胞表达和分泌蛋白（RANTES）mRNA的表达。雌激素和孕激素诱导子宫内生产的所有四种趋化因子，并可能通过自分泌/旁分泌活动的IL-1。这些数据表明，C-C趋化因子在妊娠期间子宫中巨噬细胞的积累中发挥作用。
Leukocytes accumulate in the pregnant mouse uterus following mating, during implantation and during placental development. Changes in leukocyte number are primarily due to recruitment from the blood, not local proliferation, but the underlying recruitment mechanisms are poorly understood. Mating-induced granulocyte and macrophage recruitment is due in part to pro-inflammatory and chemotactic factors present in seminal plasma. Accumulation of macrophages later in pregnancy appears to be caused in part by ovarian hormone-stimulated CSF-1 production and in part by other as yet unidentified uterine chemotactic factors. The current study was performed to assess chemokine production in the uterus during pregnancy. Northern blotting was used to demonstrate NSI/KC (KC), macrophage chemotactic protein-1 (MCP-1), macrophage inflammatory protein one α (MIP1α) and regulated inactivation, normal T expressed and secreted protein (RANTES) mRNA in the uterus. Oestrogen and progesterone induced intrauterine production of all four chemokines and may have done so through the autocrine/paracrine activities of IL-1. The data suggest that C-C chemokines play a role in accumulation of macrophages in the uterus during pregnancy.