Simultaneous quantitative determination of 9 active components in traditional Chinese medicinal preparation ShuangDan oral liquid by RP-HPLC coupled with photodiode array detection
Simultaneous quantitative determination of 9 active components in traditional Chinese medicinal preparation ShuangDan oral liquid by RP-HPLC coupled with photodiode array detection
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DOI：
10.1016/j.jpba.2011.07.016
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发表时间：
2011-12-05
影响因子：
3.4
通讯作者：
Wang, Jian-Bo
中科院分区：
文献类型：
--
作者：
Li, Hua;Wang, Si-Wang;Wang, Jian-Bo
一种简便、准确、可靠的同时分离测定9种有效成分的方法（丹参素、原儿茶酸、原儿茶醛、咖啡酸、迷迭香酸、丹参素B、丹皮酚、采用反相高效液相色谱-光电二极管阵列（PDA）联用技术对双丹口服液中芍药苷和没食子酸的含量进行了测定。侦测采用SinoChrom ODS BP C-18色谱柱，以甲醇（A）和3%冰醋酸水溶液（B）为流动相，梯度洗脱，流速为1.0 mL min（-1），PDA检测。在研究的浓度范围内观察到良好的线性行为，所有分析物的r（2）值均大于0.9992。3个浓度水平的回收率为98.21%~ 101.82%，相对标准偏差为0.07%~ 1.37%。该方法可同时测定双丹口服液中9种有效成分的含量，为双丹口服液的质量控制提供了依据。(C)2011 Elsevier B. V.保留所有权利。
A simple, accurate and reliable method for the simultaneous separation and determination of 9 active components (danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid, rosmarinic acid, salvianolic acid B, paeonol, paeoniflorin and gallic acid) in traditional Chinese medicinal preparation ShuangDan (SD) oral liquid was developed using reverse phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array (PDA) detection. The chromatographic separation was performed on a SinoChrom ODS-BP C-18 column with gradient elution using methanol (A) and 3% glacial acetic acid aqueous solution (B) at a flow rate of 1.0 mL min(-1), and with a PDA detection. Good linear behaviors over the investigated concentration ranges were observed with the values of r(2) higher than 0.9992 for all the analytes. The recoveries and relative standard deviation (RSD), measured at three concentration levels, varied from 98.21% to 101.82% and 0.07% to 1.37%, respectively. The proposed method enables the simultaneous identification and determination of 9 active components in a single run for the quality control of ShuangDan oral liquid. (C) 2011 Elsevier B.V. All rights reserved.