Antimicrobial and osteogenic properties of a hydrophilic-modified nanoscale hydroxyapatite coating on titanium.
Antimicrobial and osteogenic properties of a hydrophilic-modified nanoscale hydroxyapatite coating on titanium.
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DOI：
10.1016/j.nano.2011.07.001
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发表时间：
2012-04
期刊：
影响因子：
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通讯作者：
A. Murakami;T. Arimoto;D. Suzuki;Misato Iwai-Yoshida;Fukunaga Otsuka;Y. Shibata;T. Igarashi;R. Kamijo;T. Miyazaki
中科院分区：
文献类型：
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作者：
A. Murakami;T. Arimoto;D. Suzuki;Misato Iwai-Yoshida;Fukunaga Otsuka;Y. Shibata;T. Igarashi;R. Kamijo;T. Miyazaki
羟基磷灰石（HA）涂层钛（Ti）通常用于植入式医疗器械。本研究考察了通过在生理缓冲溶液中放电制备的纳米级HA涂层钛上的体外成骨细胞基因表达和对牙龈上菌斑早期和晚期定植者的抗菌活性。HA涂覆的Ti表面显示出超亲水性，而致密烧结的HA和单独的Ti表面显示出较低的亲水性。与裸钛表面相比，烧结HA和HA涂层钛表面增强了成骨细胞表型。HA涂层Ti能够对龈上菌斑的早期定植菌（即缓症链球菌和戈登链球菌）产生抗菌活性。这种抗微生物活性可能是由表面亲水性引起的，从而导致HA涂覆的Ti表面和细菌细胞膜之间的排斥力。相反，烧结的HA样品容易受到微生物的感染。因此，经改性的HA涂层钛可能具有用于植入式医疗器械的潜力。来自临床编辑：本研究确定，植入器械的羟基磷灰石（HA）涂层钛（Ti）表面可能会产生最佳微环境，以控制和预防感染，并可能具有潜在的未来临床应用。
Hydroxyapatite (HA)-coated titanium (Ti) is commonly used for implantable medical devices. This study examined in vitro osteoblast gene expression and antimicrobial activity against early and late colonizers of supra-gingival plaque on nanoscale HA-coated Ti prepared by discharge in a physiological buffered solution. The HA-coated Ti surface showed super-hydrophilicity, whereas the densely sintered HA and Ti surfaces alone showed lower hydrophilicity. The sintered HA and HA-coated Ti surfaces enhanced osteoblast phenotypes in comparison with the bare Ti surface. The HA-coated Ti enabled antimicrobial activity against early colonizers of supra-gingival plaques, namely Streptococcus mitis and Streptococcus gordonii. Such antimicrobial activity may be caused by the surface hydrophilicity, thereby leading to a repulsion force between the HA-coated Ti surface and the bacterial cell membranes. On the contrary, the sintered HA sample was susceptible to infection of microorganisms. Thus, hydrophilic-modified HA-coated Ti may have potential for use in implantable medical devices. FROM THE CLINICAL EDITOR: This study establishes that Hydroxyapatite (HA)-coated titanium (Ti) surface of implanted devices may result in an optimal microenvironment to control and prevent infections and may have potential future clinical applications.