Stability of Polyethylene Glycol and Zwitterionic Surface Modifications in PDMS Microfluidic Flow Chambers
Stability of Polyethylene Glycol and Zwitterionic Surface Modifications in PDMS Microfluidic Flow Chambers
复制标题
DOI：
10.1021/acs.langmuir.7b03095
复制
发表时间：
2018-01-09
期刊：
影响因子：
3.9
通讯作者：
Potkay, Joseph A.
中科院分区：
文献类型：
--
作者：
Plegue, Thomas J.;Kovach, Kyle M.;Potkay, Joseph A.
血液与材料的相互作用对于血液接触装置的使用寿命、安全性和整体成功至关重要。亲水性聚合物涂层已被用来保护血液接触材料免受自然异物反应（主要是凝血级联的内在途径）的影响，从而提高设备的使用寿命。这些涂层能够排斥蛋白质、细胞、细菌和其他微生物。希望涂层具有长期稳定性，以便获得持久的非血栓形成和防污效果。不幸的是，目前研究其在生理环境中动态流动条件下稳定性的研究有限。此外，文献中缺乏多种涂层之间的直接比较。在这项研究中，我们研究了聚乙二醇 (PEG)、两性离子磺基甜菜碱硅烷 (SBSi) 和两性离子聚乙二醇磺基甜菜碱硅烷 (PEG-SBSi) 在环境、静态流体（无流动）和生理相关流动条件下通过室温连续流动化学工艺接枝到聚二甲基硅氧烷 (PDMS) 上随时间的稳定性，并将结果与未涂层 PDMS 对照进行比较。 PEG、SBSi 和 PEG-SBSi 涂层在环境条件下可保持接触角低于 20 度长达 35 天。静态条件下 7 天后，SBSi 和 PEG-SBSi 显示出稳定性和亲水性增加。他们还保留了接触角
Blood-material interactions are crucial to the lifetime, safety, and overall success of blood contacting devices. Hydrophilic polymer coatings have been employed to improve device lifetime by shielding blood contacting materials from the natural foreign body response, primarily the intrinsic pathway of the coagulation cascade. These coatings have the ability to repel proteins, cells, bacteria, and other micro-organisms. Coatings are desired to have long-term stability, so that the nonthrombogenic and nonfouling effects gained are long lasting. Unfortunately, there exist limited studies which investigate their stability under dynamic flow conditions as encountered in a physiological setting. In addition, direct comparisons between multiple coatings are lacking in the literature. In this study, we investigate the stability of polyethylene glycol (PEG), zwitterionic sulfobetaine silane (SBSi), and zwitterionic polyethylene glycol sulfobetaine silane (PEG-SBSi) grafted by a room temperature, sequential flow chemistry process on polydimethylsiloxane (PDMS) over time under ambient, static fluid (no flow), and physiologically relevant flow conditions and compare the results to uncoated PDMS controls. PEG, SBSi, and PEG-SBSi coatings maintained contact angles below 20 degrees for up to 35 days under ambient conditions. SBSi and PEG-SBSi showed increased stability and hydrophilicity after 7 days under static conditions. They also retained contact angles