Polydopamine-Mediated Carrier with Stabilizing and Self-Antioxidative Properties for Polyphenol Delivery Systems
Polydopamine-Mediated Carrier with Stabilizing and Self-Antioxidative Properties for Polyphenol Delivery Systems
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用于多酚输送系统的具有稳定和自我抗氧化特性的聚多巴胺介导的载体
DOI：
10.1021/acs.iecr.7b04070
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发表时间：
2018
影响因子：
4.2
通讯作者：
Jiang Yanbin
中科院分区：
文献类型：
--
作者：
Li Shaomin;Li Zhixian;Pang Jiafeng;Chen Jing;Wang Hongdi;Xie Qiuling;Jiang Yanbin
本研究报道了一种简便的方法来构建通用的玉米醇溶蛋白-聚多巴胺-酪蛋白核壳纳米复合材料（ZPCs）的多酚传递系统。玉米醇溶蛋白颗粒的表面首先用贻贝启发的聚多巴胺（PDA）进行修饰，然后用酪蛋白包覆，形成稳定的胶体颗粒，以抵抗包括pH、盐度、储存、再分散和紫外线照射的环境应力。与游离白藜芦醇（RES）相比，包封RES到ZPC（RES-ZPC）提高约5倍和2.5倍的抗氧化活性DPPH和ABTS测定，分别。RES-ZPCs的细胞H2 O2清除试验显示100%的外源性活性氧清除，而游离RES仅清除63.5%。体外细胞摄取和细胞毒性实验表明，ZPCs可被细胞摄取，对细胞增殖无毒性。这种将PDA和多酚与抗氧化活性和稳定性相结合的概念可能为设计高活性、稳定的多酚递送系统提供新的见解，并有望在食品、膳食补充剂和制药行业中应用。
This study reports a facile approach to construct versatile zein-polydopamine-casein core–shell nanocomposites (ZPCs) for polyphenol delivery systems. The surface of zein particles was first modified with mussel-inspired polydopamine (PDA), and then coated with casein, forming stable colloidal particles against environmental stresses including pH, salinity, storage, redispersion, and UV irradiation. Compared with free resveratrol (RES), the encapsulation of RES into ZPCs (RES-ZPCs) enhanced approximately 5-fold and 2.5-fold antioxidant activity for DPPH and ABTS assays, respectively. Cellular H2O2scavenging assay of RES-ZPCs presents 100% exogenous reactive oxygen species elimination, whereas free RES eliminates only 63.5%. In vitro cell uptake and cytotoxicity assays show that ZPCs could be uptake and exhibits nontoxic on the cell proliferation. This concept of combining PDA and polyphenols with antioxidant activity and stability may provide new insights into designing highly active, stable polyphenol delivery systems and holds promise for applications in food, dietary supplement, and pharmaceutical industries.