SENSILLUM-LYMPH PROTEINS FROM ANTENNAL OLFACTORY HAIRS OF THE MOTH ANTHERAEA-POLYPHEMUS (SATURNIIDAE)
SENSILLUM-LYMPH PROTEINS FROM ANTENNAL OLFACTORY HAIRS OF THE MOTH ANTHERAEA-POLYPHEMUS (SATURNIIDAE)
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DOI：
10.1016/0020-1790(87)90093-x
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发表时间：
1987-01-01
期刊：
影响因子：
--
通讯作者：
KLEIN, U
中科院分区：
文献类型：
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作者：
KLEIN, U
通过微型超薄层聚丙烯酰胺凝胶中的等电聚焦和微梯度 PAGE (.mu.PAGE) 分析了雄性和雌性天蛾 Antheraea polyphemus 触角中的可溶性蛋白质。比较了雄性触角嗅毛的感器淋巴的不同制剂（分离的感器淋巴液滴、分离的毛发的冲洗液）以及雄性和雌性触角的匀浆。信息素结合蛋白 PBP 和信息素降解感器酯酶 SE 仅在雄性触角制剂中检测到。分离的感器淋巴在亮蓝染色中表现出两种蛋白质：（1）PBP呈双条带，相对分子质量Mr<14,400（α-乳白蛋白）； (2)在非常小的分子量(Mr.mchlt.14,000)处检测到一条微弱的带状寡肽。分离的感器淋巴中的 PBP 与分离毛发的冲洗溶液和雄性分支匀浆中的 PBP 相同。从分支匀浆中纯化的 PBP 显示出第三个稍小的条带。在还原SDS-μPAGE中，来自所有制品的变性PBP仅显示一条Mr为14,400的条带。 PBP的等电点(pI)为4.7。 PBP 似乎是一种非糖基化蛋白质。男女血淋巴中的糖蛋白以不同的形式存在。感器-淋巴液滴中PBP的浓度为.apprxeq。 10 mM，对应于.apprxeq的总量。一根天线中有 9 .mu.g/55 .mu.l 感器淋巴液。感器淋巴中的第三种蛋白质，即感器酯酶，仅通过酶染色可见，其 Mr 为 55,000，pI 为 3.0。在雄性和雌性触角中发现的外皮酯酶的 Mr 为 65,000，pI 5.85，Mr 为 90,000，pI 5.0。从男女的体鳞来看，100,000 Mr 范围内的酯酶溶解了 0.01%。 Triton 缓冲液可以在 .mu.PAGE 中进行演示。
The soluble proteins in the antennae of the male and female saturniid moth Antheraea polyphemus were analyzed by isoelectric focusing in miniature ultrathin-layer polyacrylamide gels and by microgradient PAGE (.mu.PAGE). Different preparations of sensillum lymph from male antennal olfactory hairs (isolated sensillum-lymph droplets, rinsing solution from isolated hairs) and homogenates of male and female antennae were compared. The pheromone-binding protein, PBP, and the pheromone-degrading sensillar esterase, SE, were detected only in male antennal preparations. Isolated sensillum lymph exhibited two proteins in Brilliant Blue staining: (1) the PBP appeared as a double band with a relative molecular mass of Mr < 14,400 (.alpha.-lactalbumin); (2) an oligopeptide was detected as a faint band at a very small molecular mass (Mr .mchlt. 14,000). The PBP in isolated sensillum lymph was identical to the PBP in the rinsing solution of isolated hairs and in male branch homogenates. Purified PBP from branch homogenates showed a third slightly smaller band. In reducing SDS-.mu.PAGE, the denatured PBP from all preparations showed only one band at a Mr of 14,400. The isoelectric point (pI) of the PBP was 4.7. The PBP appeared to be a non-glycosylated protein. Glycoproteins were present in the hemolymph in different patterns in both sexes. The concentration of PBP in sensillum-lymph droplets was .apprxeq. 10 mM, corresponding to a total amount of .apprxeq. 9 .mu.g/55 .mu.l sensillum lymph in one antenna. A third protein in the sensillum lymph, the sensillar esterase, was only visible by enzyme staining and had a Mr of 55,000 and a pI of 3.0. The integumental esterases found in male and female antennae had a Mr of 65,000, pI 5.85, and Mr 90,000, pI 5.0. From body scales of both sexes, esterases in the range of Mr 100,000 were solubilized by 0.01%. Triton buffer and could be demonstrated in .mu.PAGE.