Cell-cycle-dependent variations in FTIR micro-spectra of single proliferating HeLa cells: Principal component and artificial neural network analysis
Cell-cycle-dependent variations in FTIR micro-spectra of single proliferating HeLa cells: Principal component and artificial neural network analysis
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DOI：
10.1016/j.bbamem.2006.04.018
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发表时间：
2006-07-01
影响因子：
3.4
通讯作者：
Diem, Max
中科院分区：
文献类型：
--
作者：
Boydston-White, Susie;Romeo, Melissa;Diem, Max
我们以前曾报道过光谱差异的细胞在不同阶段的真核细胞分裂周期。这些差异是由于细胞增殖引起的剧烈的生物化学和形态学变化。我们关联这些变化的FTIR吸收和拉曼光谱的个别细胞与他们的生化年龄（或在细胞周期中的阶段），确定通过inummohistochemical染色检测的出现（和随后的消失）的细胞周期特异性细胞周期蛋白，和/或DNA合成的发生。一旦光谱与他们的细胞的染色模式相关联，我们使用多元统计的方法来分析细胞光谱的变化作为细胞周期阶段的函数。(c)2006 Elsevier B. V.保留所有权利。
We have previously reported spectral differences for cells at different stages of the eukaryotic cell division cycle. These differences are due to the drastic biochemical and morphological changes that occur as a consequence of cell proliferation. We correlate these changes in FTIR absorption and Raman spectra of individual cells with their biochemical age (or phase in the cell cycle), determined by inummohistochemical staining to detect the appearance (and subsequent disappearance) of cell-cycle-specific cyclins, and/or the occurrence of DNA synthesis. Once spectra were correlated with their cells' staining patterns, we used methods of multivariate statistics to analyze the changes in cellular spectra as a function of cell cycle phase. (c) 2006 Elsevier B.V. All rights reserved.