SUCCESSFUL TRANSPLANTATION OF MOTONEURONS INTO THE PERIPHERAL NERVE DEPENDS ON THE NUMBER OF TRANSPLANTED CELLS
SUCCESSFUL TRANSPLANTATION OF MOTONEURONS INTO THE PERIPHERAL NERVE DEPENDS ON THE NUMBER OF TRANSPLANTED CELLS
复制标题
运动神经元成功移植到周围神经取决于移植细胞的数量
DOI：
10.18999/nagjms.77.1-2.253
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发表时间：
2015
影响因子：
0.9
通讯作者：
H. Hirata
中科院分区：
文献类型：
--
作者：
Shuichi Kato;S. Kurimoto;Tomonori Nakano;H. Yoneda;H. Ishii;Satoka MITA;H. Hirata
摘要 将运动神经元 (MN) 移植到周围神经中，为肌肉神经支配提供神经元来源，称为运动神经元整合横纹肌 (MISM)，与计算机编程的功能性电刺激 (FES) 相结合，可以提供恢复功能性肌肉活动的潜力。通过比较两组，一组移植 2 × 105 个细胞（A 组），另一组移植 1 × 106 个细胞（B 组），研究恢复成年 Fischer 344 大鼠失神经肌肉神经支配所需的 MN 数量。移植后十二周，进行电生理分析、肌肉功能分析和组织分析。 B 组平均运动神经传导速度较快（12.4±1.0 m/s 比 8.5±0.7 m/s，P=0.011），复合肌肉动作电位平均振幅较大（1.6±0.4 mV 比 0.7±0.2 mV，P=0.034）。 75 ± 8°，P = 0.02）。 B 组腓神经的平均有髓轴突数量和重新受神经支配的运动终板的比例也较高（分别为 317 ± 33 vs. 104 ± 17、87.5 ± 3.4% vs. 40.6 ± 7.7%；P < 0.01）。当足够的 MN 被移植到周围神经时，MISM 形成功能性运动单位。 MISM 与 FES 相结合，为瘫痪肌肉提供了一种新的治疗策略。
ABSTRACT Transplantation of motoneurons (MN) into the peripheral nerve to provide a source of neurons for muscle reinnervation, termed motoneuron integrated striated muscle (MISM), may provide the potential to restore functional muscle activity, when combined with computer-programmed functional electrical stimulation (FES). The number of MNs required to restore innervation to denervated muscles in adult Fischer 344 rats was investigated by comparing two groups, one transplanted with 2 × 105 cells (group A) and the other with 1 × 106 cells (group B). Twelve weeks after transplantation, electrophysiological analysis, muscle function analysis, and tissue analysis were performed. The mean motor nerve conduction velocity was faster (12.4 ± 1.0 m/s vs. 8.5 ± 0.7 m/s, P = 0.011) and the mean amplitude of compound muscle action potential was larger (1.6 ± 0.4 mV vs. 0.7 ± 0.2 mV, P = 0.034) in group B. The dorsiflexed ankle angle was larger in group B (27 ± 5° vs. 75 ± 8°, P = 0.02). The mean myelinated axon number in the peroneal nerve and the proportion of reinnervated motor end plates were also greater in group B (317 ± 33 vs. 104 ± 17, 87.5 ± 3.4% vs. 40.6 ± 7.7%; P < 0.01, respectively). When sufficient MNs are transplanted into the peripheral nerve, MISM forms functional motor units. MISM, in conjunction with FES, provides a new treatment strategy for paralyzed muscles.