TRANSPORT OF METHIONINE AND PROLINE BY RAT-LIVER SLICES AND EFFECT OF CERTAIN HORMONES
TRANSPORT OF METHIONINE AND PROLINE BY RAT-LIVER SLICES AND EFFECT OF CERTAIN HORMONES
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DOI：
10.1042/bj1360015
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发表时间：
1973-01-01
影响因子：
4.1
通讯作者：
PURKISS, P
中科院分区：
文献类型：
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作者：
CRAWHALL, JC;PURKISS, P
1.本文研究了L-蛋氨酸和L-脯氨酸在离体大鼠肝切片中的转运特性。2.获得了甲硫氨酸的细胞内浓度梯度。3.碘乙酸、二硝基苯酚、无Na+培养基以及甘氨酸、赖氨酸、半胱氨酸和二硫苏糖醇均抑制甲硫氨酸摄取，但α-氨基异丁酸不抑制甲硫氨酸摄取。4.切片中蛋氨酸代谢速率缓慢。5.嘌呤霉素抑制蛋氨酸掺入蛋白质，但不蛋氨酸摄取。6.蛋氨酸抑制α-氨基异丁酸的转运，但不抑制胱氨酸的转运。7.研究了蛋氨酸的流出和交换扩散。8.甲状腺切除对大鼠肝片氨基酸转运无影响。9.添加胰岛素、胰高血糖素、肾上腺素或皮质醇不影响蛋氨酸的转运。10.在某些实验中，加入6-N，2′-O-二丁酰腺苷3′：5′-环磷酸在120分钟孵育期后增加了蛋氨酸的转运。11.脯氨酸转运的研究是无效的，因为CO2从底物的快速演变。
1. Transport characteristics ofl-methionine andl-proline in rat liver slicesin vitrowere studied. 2. Intracellular concentration gradients for methionine were obtained. 3. Methionine uptake was inhibited by iodoacetate, dinitrophenol, Na+-free media and also by glycine, lysine, cysteine and dithiothreitol but not by α-aminoisobutyrate. 4. The rate of methionine metabolism in the slice was slow. 5. Puromycin inhibited methionine incorporation into protein, but not methionine uptake. 6. Methionine inhibited the transport of α-aminoisobutyrate but not of cystine. 7. Efflux and exchange diffusion of methionine was studied. 8. Amino acid transport in rat liver slices was not affected by thyroidectomy. 9. Addition of insulin, glucagon, adrenaline or cortisol did not affect the transport of methionine. 10. Addition of 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate increased methionine transport after a 120min incubation period in some experiments. 11. Studies ofl-proline transport were invalidated because of the rapid evolution of CO2from the substrate.