Efficient binding of NC2•TATA-binding protein to DNA in the absence of TATA
Efficient binding of NC2•TATA-binding protein to DNA in the absence of TATA
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DOI：
10.1074/jbc.m406343200
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发表时间：
2005-02-18
影响因子：
4.8
通讯作者：
Meisterernst, M
中科院分区：
文献类型：
--
作者：
Gilfillan, S;Stelzer, G;Meisterernst, M
负辅因子2（NC 2）与启动子上的TATA结合蛋白（TBP）形成稳定的复合物。这阻止了转录因子（TF）IIA和TFIIB的组装，并导致RNA聚合酶II转录的抑制。在这里，我们重新审视了NC 2-TBP与DNA的相互作用。我们表明，NC 2-TBP复合物表现出显着降低的偏好TATA盒序列相比，TBP和TBP-TFIIA复合物。在染色质免疫沉淀中，发现NC 2存在于多种含TATA和无TATA的人启动子上。大量的NC 2存在于与TBP的复合物中，在大量染色质中。NC 2-TBP的复合物显示出与35-bp主要晚期启动子寡核苷酸相似的2 × 10（-9）M的DNA的K-D。在优先识别与启动子结合的TBP的同时，NC 2还加速TBP与启动子的结合并稳定TBP-DNA复合物。我们的数据表明，NC 2控制TBP结合和DNA上的维护，这在很大程度上是独立的一个典型的TATA序列。
Negative cofactor 2 (NC2) forms a stable complex with TATA-binding protein (TBP) on promoters. This prevents the assembly of transcription factor (TF) IIA and TFIIB and leads to repression of RNA polymerase II transcription. Here we have revisited the interactions of NC2-TBP with DNA. We show that NC2-TBP complexes exhibit a significantly reduced preference for TATA box sequences compared with TBP and TBP-TFIIA complexes. In chromatin immunoprecipitations, NC2 is found on a variety of human TATA-containing and TATA-less promoters. Substantial amounts of NC2 are present in a complex with TBP in bulk chromatin. A complex of NC2-TBP displays a K-D for DNA of similar to2 X 10(-9) M for a 35-bp major late promoter oligonucleotide. While preferentially recognizing promoter-bound TBP, NC2 also accelerates TBP binding to promoters and stabilizes TBP-DNA complexes. Our data suggest that NC2 controls TBP binding and maintenance on DNA that is largely independent of a canonical TATA sequence.