Characterization of a cell death-inducing endonuclease-like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae).
Characterization of a cell death-inducing endonuclease-like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae).
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DOI：
10.1002/ps.6011
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发表时间：
2021-01
影响因子：
4.1
通讯作者：
Ye, Gongyin
中科院分区：
文献类型：
--
作者：
Wang, Jiale;Yan, Zhichao;Xiao, Shan;Wang, Beibei;Fang, Qi;Schlenke, Todd;Ye, Gongyin
寄生蜂是防治害虫的重要天敌。为了确保成功的寄生，这些黄蜂将毒液沿着它们的卵注入宿主体内或身上。寄生蜂毒液调节宿主的行为、发育、代谢和免疫反应。Pteromalus puparum是一种蛹内寄生蜂，寄生于许多蝴蝶，包括世界性害虫菜粉蝶。蛹虫草毒对宿主血细胞有多种作用，包括改变血细胞的绝对和相对计数，抑制血细胞的铺展和包囊。具体地，蛹虫草毒液在体内和体外引起血细胞死亡。使用分析引导的色谱法，鉴定了诱导细胞死亡的毒液级分，并将其定义为蛹虫草核酸内切酶样毒液蛋白（PpENVP）。它属于DNA/RNA非特异性内切酶家族，含有两个保守的内切酶激活位点。我们分析了它的表达谱，并证明PpENVP依赖于两个激活位点抑制转染细胞中的基因表达。然而，PpENVP的RNA干扰并没有显著降低P. puparum毒液的细胞毒性，这表明PpENVP可能不是唯一存在的细胞毒性因子。我们的研究结果提供了新的洞察P. puparum毒液鸡尾酒的功能，并确定了一个有前途的杀虫剂候选核酸内切酶，靶向昆虫血细胞。
Parasitoid wasps are valuable natural enemies for controlling pests. To ensure successful parasitism, these wasps inject venoms along with their eggs that are deposited either into or on their hosts. Parasitoid venoms regulate host behaviors, development, metabolism, and immune responses. Pteromalus puparum is a pupal endoparasitoid that parasitizes a number of butterflies, including the worldwide pest cabbage butterfly, Pieris rapae. Venom from P. puparum has a variety of effects on host hemocytes, including alteration of absolute and relative hemocyte counts, and inhibition of hemocyte spreading and encapsulation. In particular, P. puparum venom causes hemocyte cell death in vivo and in vitro. Using assay-guided chromatography, a cell death-inducing venom fraction was identified and defined as P. puparum endonuclease-like venom protein (PpENVP). It belongs to the DNA/RNA non-specific endonuclease family, which contains two conserved endonuclease activation sites. We analyzed its expression profiles and demonstrated that PpENVP inhibits gene expression in transfected cells relying on two activation sites. However, RNA interference of PpENVP did not significantly reduce P. puparum venom cytotoxicity, suggesting that PpENVP may not be the sole cytotoxic factor present. Our results provide novel insight into the function of the P. puparum venom cocktail and identify a promising insecticide candidate endonuclease that targets insect hemocytes.
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