Long noncoding RNA DNAJC3-AS1 promotes osteosarcoma progression via its sense-cognate gene DNAJC3
Long noncoding RNA DNAJC3-AS1 promotes osteosarcoma progression via its sense-cognate gene DNAJC3
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长非编码RNA DNAJC3-AS1通过其正义同源基因DNAJC3促进骨肉瘤进展
DOI：
10.1002/cam4.1955
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发表时间：
2019-02-01
期刊：
影响因子：
4
通讯作者：
Li, Qingchu
中科院分区：
文献类型：
--
作者：
Liang, Ridong;Liu, Zezheng;Li, Qingchu
长链非编码RNA在肿瘤的发生、发展中起着重要作用。本研究以DNAJC 3-AS 1为研究对象，探讨其在骨肉瘤中的生物学功能及临床意义。检测30例骨肉瘤、癌旁组织及骨肉瘤细胞系中DNAJC 3-AS 1的表达，分析其与临床病理因素的关系。我们发现DNAJC 3-AS 1在骨肉瘤中表达上调。DNAJC 3-AS 1高表达与高分化程度（P = 0.018）和Enneking分期（P = 0.016）相关。机制上，DNAJC 3-AS 1在体外和体内增强细胞增殖、迁移和侵袭，并降低骨肉瘤对顺铂的敏感性。DNAJC 3-AS 1的这些作用通过下调其有义同源基因DNAJC 3而逆转。因此，DNAJC 3-AS 1通过调控DNAJC 3促进骨肉瘤的发生和发展，可能成为骨肉瘤的生物标志物和治疗靶点。
Long noncoding RNAs have been proved to play essential roles in tumor development and progression. In this study, we focused on DNAJC3-AS1 and investigated its biological function and clinical significance in osteosarcoma. We detected the expression of DNAJC3-AS1 in 30 pairs of matched osteosarcoma and adjacent nontumorous specimens and osteosarcoma cell lines and analyzed association between DNAJC3-AS1 levels and clinicopathological factors. We found that DNAJC3-AS1 expression was up-regulated in osteosarcoma. High level of DNAJC3-AS1 was correlated with high differentiated degree (P = 0.018) and advanced Enneking stage (P = 0.016). Mechanistically, DNAJC3-AS1 enhanced cell proliferation, migration, and invasion in vitro and in vivo and reduced sensitivity of osteosarcoma to cisplatin. These effects of DNAJC3-AS1 were reversed by down-regulation of its sense-cognate gene DNAJC3. Thus, DNAJC3-AS1 promotes osteosarcoma development and progression by regulating DNAJC3 and might be a biomarker and therapeutic target for osteosarcoma.