ULTRASTRUCTURAL OBSERVATIONS OF BOAR SPERMATOZOA PENETRATING ZONA-FREE HAMSTER EGGS
ULTRASTRUCTURAL OBSERVATIONS OF BOAR SPERMATOZOA PENETRATING ZONA-FREE HAMSTER EGGS
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DOI：
10.1095/biolreprod23.2.481
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发表时间：
1980-01-01
影响因子：
3.6
通讯作者：
IRITANI, A
中科院分区：
文献类型：
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作者：
IMAI, H;NIWA, K;IRITANI, A
在改良的 Krebs-Ringer 碳酸氢盐培养基中预孵育的公猪精子从未穿透无透明带的仓鼠卵，并且在其顶体中未观察到结构变化。在成熟母猪离体子宫中预培养 5 小时的精子顶体帽区域观察到顶体反应。只有顶体反应的精子与无透明带的仓鼠卵融合。精卵膜融合与其他物种的同源受精基本相同；只有顶体后区域周围的卵子微绒毛与精子质膜融合。仓鼠卵中精子原核的超微结构特征与猪卵中的不同，表明某些卵黄因子可能参与精子原核的形成。
Boar spermatozoa preincubated in a modified Krebs-Ringer bicarbonate medium never penetrated zona-free hamster eggs, and no structural changes were observed in their acrosomes. The acrosome reaction was observed at the acrosomal cap region of spermatozoa preincubated for 5 h in the isolated uterus from a maturing gilt. Only acrosome-reacted spermatozoa fused with zona-free hamster eggs. Sperm-egg membrane fusion was essentially the same as that of homologous fertilization in other species; only the microvilli of the egg surrounding the postacrosomal region fused with sperm plasma membrane. The ultrastructural features of the sperm pronucleus in the hamster egg were dissimilar to those in the pig egg, indicating that some vitelline factor might be involved in the formation of the sperm pronucleus.