Developmentally controlled expression of immunoglobulin VH genes.
Developmentally controlled expression of immunoglobulin VH genes.
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DOI：
10.1126/science.3975629
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发表时间：
1985-03
期刊：
影响因子：
56.9
通讯作者：
R. Perlmutter;J. Kearney;S. P. Chang;L. Hood
中科院分区：
文献类型：
--
作者：
R. Perlmutter;J. Kearney;S. P. Chang;L. Hood
虽然抗体的多样性主要来自于作用于有限数量的种系抗体基因的明显随机组合和体细胞突变机制，但抗体库在哺乳动物个体发育期间以有序的方式发展。一系列的早期前B和B淋巴细胞系进行了检查，以确定是否有序重排的免疫球蛋白重链（VH）的可变区的基因家族可能是抗体反应的程序化发展的基础。结果表明，胎儿B系细胞的VH库在很大程度上限于VH 7183基因家族，并且在新生儿发育期间发生额外的VH基因家族的随后招募。这些结果对理解免疫功能的个体发生具有重要意义。
Although antibody diversity arises mainly from apparently random combinatorial and somatic mutational mechanisms acting upon a limited number of germline antibody genes, the antibody repertoire develops in an ordered fashion during mammalian ontogeny. A series of early pre-B and B-lymphocyte cell lines were examined to determine whether an ordered rearrangement of gene families of the variable region of immunoglobulin heavy chains (VH) may be the basis for the programmed development of the antibody response. The results indicated that the VH repertoire of fetal B-lineage cells is largely restricted to the VH 7183 gene family and that subsequent recruitment of additional VH gene families occurs during neonatal development. These results have important implications in understanding the ontogeny of immune function.