Applying genomics to the avian inner ear: Development of subtractive cDNA resources for exploring sensory function and hair cell regeneration
Applying genomics to the avian inner ear: Development of subtractive cDNA resources for exploring sensory function and hair cell regeneration
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DOI：
10.1016/j.ygeno.2005.12.014
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发表时间：
2006-06-01
期刊：
影响因子：
4.4
通讯作者：
Lovett, Michael
中科院分区：
文献类型：
--
作者：
Hawkins, R. David;Helms, Cynthia A.;Lovett, Michael
我们应用基于micro-cDNA的消减方法来鉴定鸡内耳再生感觉上皮（SE）中表达的基因。感觉毛细胞在鸟类椭圆囊SE是在一个恒定的周转状态，其中死亡的毛细胞被取代的新的来自支持细胞。相比之下，耳蜗中的毛细胞保持静止，除非受损。我们利用这种差异来富集胞囊特异性基因，使用迭代cDNA减法并证明富集胞囊特异性序列。总共1710个cDNA序列读数揭示了许多编码SE的已知结构组分（例如，Harmonin和β-tectorin）、参与细胞增殖的蛋白质（例如P311、HIPK 2和SPALT 1）以及许多其他未知功能的cDNA的存在。这些文库是同类文库中的第一个，应该证明对发现听力障碍、再生和凋亡途径以及新型鸡EST的候选基因是有用的。(c)2006年爱思唯尔公司All rights reserved.
We applied a micro-cDNA-based subtraction method to identify genes expressed in the regenerating sensory epithelia (SE) of the chicken inner ear. Sensory hair cells in the avian utricle SE are in a constant state of turnover, where dying hair cells are replaced by new ones derived from supporting cells. In contrast, hair cells in the cochlea remain quiescent unless damaged. We used this difference to enrich for utricle-specific genes, using reiterative cDNA subtraction and demonstrate enrichment for utricle-specific sequences. A total of 1710 cDNA sequence reads revealed the presence of many cDNAs encoding known structural components of the SE (for example, Harmonin and beta-tectorin), proteins involved in cellular proliferation, such as P311, HIPK2, and SPALT1, among many others of unknown function. These libraries are the first of their kind and should prove useful for the discovery of candidate genes for hearing disorders, regenerative and apoptotic pathways, and novel chicken ESTs. (c) 2006 Elsevier Inc. All rights reserved.