A site on rod G protein alpha subunit that mediates effector activation.
A site on rod G protein alpha subunit that mediates effector activation.
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杆状 G 蛋白 α 亚基上介导效应器激活的位点。
DOI：
10.1126/science.1317058
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hamm,HE
中科院分区：
文献类型：
--
作者：
Rarick,HM;Artemyev,NO;Hamm,HE
异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）由感觉或激素受体激活。反过来，G蛋白激活效应蛋白，如腺苷酸环化酶，环鸟苷3′，5 ′-磷酸磷酸二酯酶（cGMP PDE），磷脂酶C，钾和钙离子通道的机制知之甚少。G蛋白转导素（αt）α亚基上的一个位点已被鉴定，该位点与cGMP磷酸二酯酶（视杆细胞中的效应酶）相互作用并激活cGMP磷酸二酯酶。一个22个氨基酸的肽，对应于αt的COOH末端区域的残基293至314，完全模拟α t并有效激活PDE。该区域与受体激活结构域相邻;因此，该G蛋白的α亚基具有与其效应子和受体相互作用的位点，该位点位于COOH末端附近。
The heterotrimeric guanine nucleotide binding proteins (G proteins) are activated by sensory or hormone receptors. In turn, the G proteins activate effector proteins such as adenylyl cyclase, cyclic guanosine 3′,5′-monophosphate phosphodiesterase (cGMP PDE), phospholipase C, and potassium and calcium ion channels by mechanisms that are poorly understood. A site on the α subunit of the G protein transducin (αt) has been identified that interacts with and activates cGMP phosphodiesterase, the effector enzyme in rod photoreceptors. A 22-amino acid peptide, corresponding to residues 293 to 314 from the COOH-terminal region of αt, fully mimicked αtand potently activated PDE. This region is adjacent to the receptor activation domain; thus, the α subunit of this G protein has a site for interaction with both its effector and receptor that maps near the COOH-terminus.