REGULATION OF MOUSE OOCYTE GROWTH - PROBABLE NUTRITIONAL ROLE FOR INTERCELLULAR COMMUNICATION BETWEEN FOLLICLE CELLS AND OOCYTES IN OOCYTE GROWTH
REGULATION OF MOUSE OOCYTE GROWTH - PROBABLE NUTRITIONAL ROLE FOR INTERCELLULAR COMMUNICATION BETWEEN FOLLICLE CELLS AND OOCYTES IN OOCYTE GROWTH
复制标题
DOI：
10.1002/jez.1402290217
复制
发表时间：
1984-01-01
影响因子：
--
通讯作者：
SCHULTZ, RM
中科院分区：
文献类型：
--
作者：
HERLANDS, RL;SCHULTZ, RM
通过 2 种不同的测定程序确定，细胞间通讯是在没有粘附卵泡细胞的小鼠卵母细胞与单层卵泡或 3T3 细胞之间体外建立的。已知这两种细胞类型都能够形成同源间隙连接，并且卵泡细胞在体内自然地与卵母细胞形成异源间隙连接。通过两种不同的细胞间通讯测定确定，通讯缺陷的单层 L [肿瘤成纤维细胞] 细胞未与卵母细胞建立细胞间通讯。在培养基中或单层L细胞上培养4天的卵母细胞直径显着减小，分别为9.7μm和13.1μm。相反，在卵泡细胞单层上培养 4 天的卵母细胞平均增加。直径4.7μm。在单层3T3细胞上培养4天的卵母细胞直径略有减小，即2.1μm。支持卵母细胞生长中卵泡细胞和卵母细胞之间的细胞间通讯的营养作用。
Intercellular communication, as determined by 2 different assay procedures, was established in vitro between mouse oocytes free of adhering follicle cells and monolayers of either follicle or 3T3 cells. Both of these cell types are known to be able to form homologous gap junctions, and follicle cells naturally form heterologous gap junctions with oocytes in vivo. Monolayers of L [neoplastic fibroblast] cells that are communication deficient did not establish intercellular communication with oocytes as determined by the 2 different assays for intercellular communication. The diameter of oocytes cultured for 4 days in medium or on monolayers of L cells decreased markedly, 9.7 and 13.1 .mu.m, respectively. In contrast, oocytes cultured for 4 days on follicle cell monolayers increased on the average .apprx. 4.7 .mu.m in diameter. Oocytes cultured for 4 days on monolayers of 3T3 cells decreased slightly in diameter, i.e., 2.1 .mu.m. A nutritional role for intercellular communication between follicle cells and oocytes in oocyte growth is supported.