Light scattering by lungs correlates with stereological measurements.
Light scattering by lungs correlates with stereological measurements.
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肺部的光散射与体视测量相关。
DOI：
10.1152/jappl.1985.58.1.97
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
HoppinJr,FG
中科院分区：
文献类型：
--
作者：
Suzuki,S;Butler,JP;Oldmixon,EH;HoppinJr,FG
光经肺组织后向散射的模式应在很大程度上取决于空气空间的大小，也同样取决于肺的内表面积。为了验证和应用这一点，我们将激光束通过胸膜表面照射到切除的肺部，并用聚焦光电探测器测量光束周围的背散射光。强度I，作为距离r的函数而下降，从光的进入点开始。I(r)曲线的构型与理论在30年的I范围内非常接近。I(r)随肺容量的变化而系统地变化。光学平均自由程λ由光学扫描后立即固定的一系列犬齿叶的I(r)曲线计算，并与平均线性截距Lm（肺泡大小指标）的立体测量结果进行比较。在高肺容量时，λ与Lm的关系与肺泡间隔反射一致。此外，在肺容量较低时，出现了大量的屈光成分。该技术独立于目前的立体学方法，具有无创、连续的优点，并且可能适用于非固定肺的动态事件。
The pattern of light backscattered by lung tissue should depend strongly on the size of air spaces and equivalently on the internal surface area of the lung. To verify and apply this, we shone a laser beam into excised lungs through the pleural surface and measured the backscattered light surrounding the beam with a focused photodetector. The intensity, I, fell off as a function of distance, r, from the point of entry of light. The configurations of I(r) curves corresponded closely to theory over a 3-decade range of I. I(r) changed systematically with lung volume. The optical mean free path, lambda, was calculated from I(r) curves in a series of canine lobes fixed immediately after optical scanning and was compared with stereological measurement of mean linear intercept, Lm, an index of alveolar size. At high lung volumes the relation of lambda to Lm was consistent with reflection by alveolar septa. At lower lung volumes there appeared to be, additionally, a substantial refractive component. This technique is independent of current stereological methods and has the advantages of being noninvasive, continuous, and potentially applicable to dynamic events in unfixed lungs.