Accentuated response to phenylhydrazine and erythropoietin in mice genetically impaired for their GATA-1 expression (GATA-1(low) mice).
Accentuated response to phenylhydrazine and erythropoietin in mice genetically impaired for their GATA-1 expression (GATA-1(low) mice).
复制标题
GATA-1 表达基因受损的小鼠（GATA-1（低）小鼠）对苯肼和促红细胞生成素的反应增强。
作者：
A. Vannucchi;Lucia Bianchi;C. Cellai;F. Paoletti;V. Carrai;A. Calzolari;L. Centurione;R. Lorenzini;Claudio Carta;E. Alfani;Massimo Sanchez;G. Migliaccio;A. Migliaccio
研究了遗传上不能上调加塔-1表达的小鼠（加塔-1（低）小鼠）对急性（苯肼[PHZ]诱导的贫血）和慢性（每只小鼠用10 U促红细胞生成素[EPO]体内处理5天）红细胞刺激的反应。成年加塔-1（低）小鼠血小板严重减少（血小板计数[x 10（9）/L] 82.0 +/- 28.0与对照同窝小鼠的840 +/- 170.0，P <0.001），但红细胞压积（Hct）正常（约为1.0的0.47比例[47%]）。这些突变体的脾脏比正常脾脏大2.5倍，并且含有5倍多的巨核细胞（4A 5（+））、红细胞（TER-119（+））和双能（红细胞/巨核细胞，TER-119（+）/4A 5（+））前体细胞。这些动物的骨髓和脾脏含有更高频率的爆发形成单位-红系细胞（BFU-E）-和集落形成单位-红系细胞（CFU-E）-衍生的集落（2倍和6倍，分别）比他们正常的同窝。加塔-1（低）小鼠从PHZ诱导的贫血中恢复的时间比其正常同窝出生的小鼠快2天（P <0.01）。作为对EPO的应答，加塔-1（低）小鼠的Hct升高至1.0的0.68比例（68%），而对照组达到1.0的0.55比例（55%）（P <0.01）。加塔-1（低）和正常小鼠对PHZ和EPO的反应相似（2- 3倍），脾的大小和细胞结构增加（增加主要限于红系的祖细胞和前体细胞）。然而，尽管类似的相对细胞增加，所有这些细胞群的增加，在绝对细胞数，在突变体中比在野生型小鼠显着更高。总之，加塔-1（低）突变增加了对红系刺激的应答的幅度，这是其脾中红系祖细胞扩增的结果。
The response of mice genetically unable to up-regulate GATA-1 expression (GATA-1(low) mice) to acute (phenylhydrazine [PHZ]-induced anemia) and chronic (in vivo treatment for 5 days with 10 U erythropoietin [EPO] per mouse) erythroid stimuli was investigated. Adult GATA-1(low) mice are profoundly thrombocytopenic (platelet counts [x 10(9)/L] 82.0 +/- 28.0 vs 840 +/- 170.0 of their control littermates, P <.001) but have a normal hematocrit (Hct) (approximately.47 proportion of 1.0 [47%]). The spleens of these mutants are 2.5-fold larger than normal and contain 5-fold more megakaryocytic (4A5(+)), erythroid (TER-119(+)), and bipotent (erythroid/megakaryocytic, TER-119(+)/4A5(+)) precursor cells. Both the marrow and the spleen of these animals contain higher frequencies of burst-forming units-erythroid (BFU-E)- and colony-forming units-erythroid (CFU-E)-derived colonies (2-fold and 6-fold, respectively) than their normal littermates. The GATA-1(low) mice recover 2 days faster from the PHZ-induced anemia than their normal littermates (P <.01). In response to EPO, the Hct of the GATA-1(low) mice raised to.68 proportion of 1.0 (68%) vs the.55 proportion of 1.0 (55%) reached by the controls (P <.01). Both the GATA-1(low) and the normal mice respond to PHZ and EPO with similar (2- to 3-fold) increases in size and cellularity of the spleen (increases are limited mostly to cells, both progenitor and precursor, of the erythroid lineage). However, in spite of the similar relative cellular increases, the increases of all these cell populations are significantly higher, in absolute cell numbers, in the mutant than in the wild-type mice. In conclusion, the GATA-1(low) mutation increases the magnitude of the response to erythroid stimuli as a consequence of the expansion of the erythroid progenitor cells in their spleen.
影响因子：
10.5
作者：
BRIEGEL, K;LIM, KC;ZENKE, M
通讯作者：
ZENKE, M
新型大鼠抗小鼠血小板单克隆抗体的特性：在巨核细胞研究中的应用。
影响因子：
2.6
作者：
Burstein,SA;Friese,P;Downs,T;Mei,RL
通讯作者：
Mei,RL
影响因子：
20.3
作者：
L. Kelley;M. Koury;M. Bondurant;S. Koury;S. Sawyer;A. Wickrema
通讯作者：
L. Kelley;M. Koury;M. Bondurant;S. Koury;S. Sawyer;A. Wickrema