Expression of β-defensins in gingival health and in periodontal disease
Expression of β-defensins in gingival health and in periodontal disease
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DOI：
10.1111/j.0904-2512.2004.00143.x
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发表时间：
2004-05-01
影响因子：
3.3
通讯作者：
Guthmiller, JM
中科院分区：
文献类型：
--
作者：
Bissell, J;Joly, S;Guthmiller, JM
背景：人β-防御素(HBDS)是上皮来源的抗菌肽。已有报道三种HBD(HBD-1、HBD-2和HBD-3)在口腔粘膜、牙龈和唾液腺中表达。然而，它们在预防口腔感染中的作用还不是很清楚。本研究检测了hBD-1、hBD-2和hBD-3mRNAs在牙周健康和牙周疾病中的表达。提取总RNA，用35周期和半定量(25周期)逆转录聚合酶链式反应(RT-PCR)分析β-防御素的表达。表达水平被分配给无表达、低表达或高表达的顺序分数。用同质性检验和Cochran-Mantel-Haenszel检验比较健康组和疾病组之间的mRNA表达；用准确度检验和Kendall‘s tau-b统计分析变量之间的相关性。结果：所有49个样本都有HBD-1、HBD-2和HBD-3的基础mRNA表达。健康组织中hBD-3的表达水平显著高于病变组织(P=0.012)。HBD-2在正常组织中的表达也较高(P=0.12)。HBD-1、hBD-2和hBD-3mRNA的表达水平相互关联(P<；0.001)。结论：在牙周病原体感染的宿主免疫反应中，hBD-3mRNA的高水平表达提示防御素具有潜在的重要保护作用。
BACKGROUND: Human beta-defensins (HBDs) are epithelial-derived antimicrobial peptides. The expression of three HBDs (HBD-1, HBD-2, and HBD-3) has been reported in oral mucosa, gingiva, and salivary glands. However, their role in protection against oral infections is not well understood. This study examined the expression of HBD-1, HBD-2, and HBD-3 mRNAs in gingival health and periodontal disease.METHODS: Gingival tissue discarded from periodontal procedures was obtained from 20 periodontally healthy and 29 periodontally diseased sites. Total RNA was isolated, and expression of beta-defensins was analyzed by both 35-cycle and semiquantitative (25-cycle) reverse transcription-polymerase chain reaction (RT-PCR). The level of expression was assigned ordinal scores of no expression, low expression, or high expression. mRNA expression was compared between healthy and diseased groups using exact tests of homogeneity and Cochran-Mantel-Haenszel tests; associations among the variables were assessed using exact tests and Kendall's tau-b statistic.RESULTS: All 49 samples demonstrated basal mRNA expression of HBD-1, HBD-2, and HBD-3. Significantly higher levels of HBD-3 (P = 0.012) expression were found in the healthy tissues as compared to the diseased ones. There was also a suggestion of higher expression of HBD-2 in the healthy tissues (P = 0.12). Levels of HBD-1, HBD-2, and HBD-3 mRNA expression were correlated with one another (P < 0.001).CONCLUSIONS: High levels of HBD-3 mRNA expression in healthy tissues suggest a potentially important protective role for defensins in the host immune response to infection by periodontal pathogens.