Injectable thioketal-containing hydrogel dressing accelerates skin wound healing with the incorporation of reactive oxygen species scavenging and growth factor release
Injectable thioketal-containing hydrogel dressing accelerates skin wound healing with the incorporation of reactive oxygen species scavenging and growth factor release
复制标题
可注射的含硫缩酮的水凝胶敷料通过结合活性氧清除和生长因子释放来加速皮肤伤口愈合
DOI：
10.1039/d1bm01179k
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发表时间：
2021-11-02
影响因子：
6.6
通讯作者：
Pei, Renjun
中科院分区：
文献类型：
--
作者：
An, Zhen;Zhang, Liwei;Pei, Renjun
伤口愈合是一个复杂的动态过程。在皮肤损伤发生过程中，过高的活性氧（ROS）水平与持续的炎症反应有关，从而限制了伤口的有效修复。尽管多功能水凝胶因其独特的优点被认为是理想的伤口敷料，但开发具有快速凝胶速率、形状适应和抗氧化功能的水凝胶敷料仍然是一个至关重要的挑战。在这项工作中，合成了一种含有硫缩酮键的 ROS 响应性可注射聚乙二醇水凝胶（PEG-TK 水凝胶），并用于递送表皮生长因子（EGF）。我们采用生物正交点击化学来交联聚合物链，以获得具有快速凝胶时间、可注射性和形状适应性的EGF@PEG-TK水凝胶。更有趣的是，PEG-TK水凝胶中的硫酮键不仅清除了伤口部位过量的ROS，而且还实现了响应性和受控的EGF释放以促进再生。 EGF@PEG-TK水凝胶治疗具有保护细胞免受氧化应激、加速伤口闭合以及减少全层皮肤缺损模型中疤痕形成的优点。这项工作为开发抗氧化水凝胶敷料以促进伤口修复提供了一种有前途的策略。
Wound healing is a complex dynamic process. During the occurrence of skin injury, the excessive reactive oxygen species (ROS) level is associated with sustained inflammatory response, which limits efficient wound repair. Although multifunctional hydrogels are considered ideal wound dressings due to their unique advantages, the development of hydrogel dressings with rapid gelling rates, shape adaptation, and antioxidant function is still a vital challenge. In this work, a ROS-responsive injectable polyethylene glycol hydrogel containing thioketal bonds (PEG-TK hydrogel) was synthesized and utilized to deliver epidermal growth factor (EGF). We adopted bio-orthogonal click chemistry for crosslinking the polymer chains to obtain the EGF@PEG-TK hydrogel with fast gelation time, injectability and shape-adaptability. More interestingly, the thioketal bonds in the PEG-TK hydrogel not only scavenged excessive ROS in the wound sites but also achieved responsive and controlled EGF release to facilitate regeneration. The EGF@PEG-TK hydrogel treatment offered the benefits of protecting cells from oxidative stress, accelerating wound closure, and reducing scar formation in the full-thickness skin defect model. This work provides a promising strategy for developing antioxidant hydrogel dressing for facilitating the repair of wounds.