Amplification units containing human N-myc and c-myc genes.
Amplification units containing human N-myc and c-myc genes.
复制标题
含有人类 N-myc 和 c-myc 基因的扩增单位。
DOI：
10.1073/pnas.83.4.1031
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Vogelstein,B
中科院分区：
文献类型：
--
作者：
Kinzler,KW;Zehnbauer,BA;Brodeur,GM;Seeger,RC;Trent,JM;Meltzer,PS;Vogelstein,B
检查了人类肿瘤中含有扩增的 myc 基因的扩增单位。所有情况下的扩增单元均由与 myc 基因本身的编码区共扩增的大基因组区域组成。在八个含有 N-myc 扩增的独立神经母细胞瘤中，扩增单位估计为 290 至 430 KB。这种放大单位在不同的神经母细胞瘤中高度保守，一些神经母细胞瘤含有几乎相同的单位。相比之下，含有 c-myc 扩增的 5 个肿瘤细胞系表现出扩增单位的大小（90 至 300 kilobase）和序列内容变化更大；至少可以辨别出三种不同模式的 c-myc 扩增单元。
The amplification units in human tumors containing amplified myc genes were examined. The amplification unit in all cases consisted of a large genomic region coamplified with the coding region of the myc genes themselves. In eight independent neuroblastomas containing N-myc amplifications, the amplification unit was estimated to be 290 to 430 kilobases. This amplification unit was highly conserved among the different neuroblastomas, with some neuroblastomas containing almost identical units. In contrast, five tumor cell lines containing c-myc amplifications exhibited amplification units that were more variable in size (90 to 300 kilobases) and sequence content; at least three different patterns of c-myc amplification units could be discerned.