Engagement of Posthemorrhagic Shock Mesenteric Lymph on CD4+ T Lymphocytes In Vivo and In Vitro
Engagement of Posthemorrhagic Shock Mesenteric Lymph on CD4+ T Lymphocytes In Vivo and In Vitro
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体内外失血性休克肠系膜淋巴对 CD4 T 淋巴细胞的参与
DOI：
10.1016/j.jss.2020.06.044
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发表时间：
2020-12-01
影响因子：
2.2
通讯作者：
Niu, Chun-Yu
中科院分区：
文献类型：
--
作者：
Jiang, Li-Na;Mi, Ya-Li;Niu, Chun-Yu
背景：免疫功能障碍与失血性休克后肠系膜淋巴回流有关。为了确定CD 4（+）T淋巴细胞的增殖和细胞因子产生能力，在失血性休克小鼠模型中评估PHSML引流对脾脏CD 4（+）T淋巴细胞的影响。将正常脾CD 4（+）T淋巴细胞与引流的正常肠系膜淋巴液（NML）、低血压时的PHSML（PHSML-H）、或复苏后0 h至3 h的PHSML（PHSML-R），以验证PHSML的直接增殖作用。失血性休克导致CD 4（+）T淋巴细胞增殖和IL-2及IL-2受体mRNA表达减少，IL-2和IFN-γ表达减少，在上清液中的IFN-γ水平。相反，白细胞介素-4水平升高。PHSML引流可逆转这些效应。此外，NML孵育可促进CD 4（+）T淋巴细胞增殖，而PHSML-H和PHSML-R处理对CD 4（+）T淋巴细胞增殖具有双相效应，表现为早期促进作用和后期抑制作用。与NML相比，PHSML-H在12 h时IL-2表达增加，而在24 h时IL-2和IFN-γ的表达均降低。相比之下，PHSML-R在24小时诱导IL-2和IFN-γ水平显著增加。PHSML-H或PHSML-R孵育12 h后，CD 4（+）T淋巴细胞中IL-4表达降低，但24 h后增加。结论：PHSML对诱导炎症反应的CD 4（+）T淋巴细胞增殖具有直接抑制作用，这与细胞免疫功能障碍有关。(C)2020爱思唯尔公司All rights reserved.
Background: Immune dysfunction is associated with posthemorrhagic shock mesenteric lymph (PHSML) return. To determine the proliferation and cytokine production capacity of CD4(+) T lymphocytes, the effect of PHSML drainage on spleen CD4(+) T lymphocytes in a mouse model of hemorrhagic shock was assessed.Methods: The normal spleen CD4(+) T lymphocytes were in vitro incubated with either drained normal mesenteric lymph (NML), PHSML during hypotension (PHSML-H), or PHSML from 0 h to 3 h after resuscitation (PHSML-R) to verify direct proliferation effects of PHSML.Results: Hemorrhagic shock led to reduction of proliferation and mRNA expression of interleukin 2 (IL-2) and IL-2 receptor in CD4(+) T lymphocytes and to decrease in IL-2 and interferon gamma (IFN-gamma) levels in supernatants. In contrast, the interleukin-4 levels were increased. These effects were reversed by PHSML drainage. Moreover, NML incubation promoted CD4(+) T lymphocyte proliferation, whereas both PHSML-H and PHSML-R treatment had a biphasic effects on CD4(+) T lymphocyte proliferation, exhibiting an enhanced effect at early stages and an inhibitory effect at later stages. Compared with NML, PHSML-H increased IL-2 expression at 12 h, but decreased expression of both IL-2 and IFN-gamma at 24 h. By contrast, PHSML-R induced significant increases in IL-2 and IFN-gamma levels at 24 h. Interleukin-4 expression in CD4(+) T lymphocytes was reduced at 12 h, but augmented at 24 h after incubation with either PHSML-H or PHSML-R.Conclusions: The results indicate that PHSML has a direct inhibitory effect on CD4(+) T lymphocyte proliferation that induces an inflammatory response, which is associated with cellular immune dysfunction. (C) 2020 Elsevier Inc. All rights reserved.