Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus.
Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus.
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DOI：
10.1292/jvms.14-0599
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Oishi K
中科院分区：
文献类型：
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作者：
Aihara N;Horiuchi N;Hikichi N;Ochiai M;Hosoda Y;Ishikawa Y;Shimazaki Y;Oishi K
传染性法氏囊病（IBD）的特征是由于萎缩的腔上囊（BF）中淋巴细胞的耗竭而引起的免疫抑制。我们有时会遇到矛盾的结果：鸡感染疫苗IBD病毒（IBDV）株，有时表现出高度萎缩的BF，但没有免疫抑制。在这项研究中，接种疫苗或IBDV野生型毒株的鸡随后接种纽卡斯尔病病毒（NDV）的B1毒株。对法氏囊变化进行组织学检查，重点检查法氏囊滤泡。同时用血凝抑制试验检测其对NDV的免疫反应性。在大体检查中，我们在疫苗株给药组（疫苗组）中观察到少数鸡出现严重萎缩的BF，严重程度与野生型菌株给药组（野生型组）相同。然而，这些鸡保留了对NDV的体液抗体应答，并显示具有比野生型组更多的囊卵泡。这些结果表明，宏观评价不能准确地反映免疫反应性和法氏囊损伤的程度，在IBDV感染鸡。我们还发现了非免疫抑制鸡在野生型组。根据我们的统计分析，这些非免疫抑制的鸡保留了显着较高数量的正常卵泡和总卵泡。此外，NDV-HI滴度和正常卵泡数之间的相关系数较高，发现在野生型组。这些结果表明，保留的正常卵泡的数量是重要的免疫反应性感染IBDV的鸡。
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine strain-administered groups (vaccine groups), and the level of severity was the same as that in the wild-type strain-administered group (wild-type group). However, these chickens retained humoral antibody responses to NDV and were revealed to possess a higher number of bursal follicles than those of the wild-type group. These results indicated that macroscopic evaluation dose not accurately reflect the immunoreactivity and degree of bursal damage in IBDV-administered chickens. We also found non-immunosuppressed chickens in the wild-type group. These non-immunosuppressed chickens retained a significantly higher number of normal follicles and total follicles according to our statistical analysis. Furthermore, a high correlation coefficient between the NDV-HI titer and the number of normal follicles was found in the wild-type group. These results implied that the retained number of normal follicles is important for the immunoreactivity of chickens infected with IBDV.