Expression of estrogen receptor alpha and beta in peritoneal and ovarian endometriosis
Expression of estrogen receptor alpha and beta in peritoneal and ovarian endometriosis
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DOI：
10.1016/s0015-0282(01)01783-6
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发表时间：
2001-06-01
影响因子：
6.7
通讯作者：
Okamura, K
中科院分区：
文献类型：
--
作者：
Matsuzaki, S;Murakami, T;Okamura, K
目的：为了定量和比较ER-α和ER-β的信使RNA（mRNA）水平卵巢增生性囊肿和红色和黑色腹膜增生性lesions.Design：前瞻性研究。设置：大学医院。患者（s）：患者有或没有endometriosis。干预（s）：腹膜（n = 33）和卵巢增生性病变（n = 37）的样本，在腹腔镜手术中获得。在手术过程中或手术后即刻获取正常在位子宫内膜组织和肉眼可见的正常腹膜组织作为对照。主要结果测量：使用实时逆转录（RT）-PCR分析、TaqMan RT-PCR和非放射性原位杂交（ISH）技术检测ER-α和ER-β的mRNA表达。结果：在位子宫内膜和异位子宫内膜组织中ER-α mRNA的表达水平明显高于ER-β mRNA。ER-α与ER-β mRNA的相对比值（ER-α/ER-β）在腹膜红色病变中显著高于黑色病变和卵巢增生性囊肿。ER-α/ER-β在增殖期在位内膜和腹膜红色病变间无显著差异。结论：ER-α在子宫内膜腺上皮细胞和间质细胞中的高表达可能是腹膜和卵巢子宫内膜异位症发生、发展的重要机制。（Fertil Steril（R）2001;75. 1198-1205. (C)2001年由美国生殖医学学会出版。
Objective: To quantify and compare messenger RNA (mRNA) levels of ER-alpha and ER-beta among ovarian endometriotic cysts and red and black peritoneal endometriotic lesions.Design: Prospective study.Setting: University hospital.Patient(s): Patients with or without endometriosis.Intervention(s): Samples of peritoneal (n = 33) and ovarian endometriotic lesions (n = 37) were obtained during laparoscopic surgery. Normal eutopic endometrial tissues and macroscopically normal peritoneal tissues were obtained as controls during or just after surgery.Main Outcome Measure(s): Expression of mRNA for ER-alpha and ER-beta, using a real-time reverse transcription (RT)-PCR assay, TaqMan RT-PCR, and nonradioactive in situ hybridization (ISH) techniques.Result(s): Both eutopic endometrium and endometriotic tissues showed predominantly higher levels of ER-alpha than ER-beta mRNA. Relative ratio of ER-alpha to ER-beta (ER-alpha /ER-beta) mRNA in red peritoneal lesions was significantly higher than in black lesions and ovarian endometriotic cysts. There was no significant difference in ER-alpha /ER-beta between proliferative eutopic endometrium and red peritoneal lesions. These results were confirmed by ISH analysis, which also revealed that the two estrogen receptors were localized in both epithelial and stromal cells of endometriotic tissues.Conclusion(s): The predominant expression of ER-alpha in both glandular epithelial and stromal cells may be essential for the development and growth of peritoneal and ovarian endometriosis. (Fertil Steril(R) 2001;75. 1198-1205. (C) 2001 by American Society for Reproductive Medicine.).