Bovine milk exosome proteome
Bovine milk exosome proteome
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DOI：
10.1016/j.jprot.2011.11.017
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发表时间：
2012-02-16
影响因子：
3.3
通讯作者：
Sacco, Randy E.
中科院分区：
文献类型：
--
作者：
Reinhardt, Timothy A.;Lippolis, John D.;Sacco, Randy E.
外泌体是40-100 nm的膜囊泡，起源于内吞，由细胞分泌，存在于包括牛奶在内的生物液体中。这些外泌体是细胞外细胞器，在细胞内通讯和免疫功能中起重要作用。因此，牛奶外泌体的蛋白质组可以为牛奶生产的复杂过程提供见解。从泌乳中期奶牛的乳中分离出外泌体。纯化的外泌体经胰蛋白酶消化，进行离线高pH反相色谱，并在连接串联质谱计的纳米色谱上进一步分离。结果鉴定出2107种蛋白，其中包括所有主要的外泌体蛋白标记。乳脂球膜（MFGM）蛋白（Butyrophilin, Xanthine oxidase， Adipophilin和Lactadherin）是乳外泌体中含量最多的蛋白。然而，它们仅占牛奶外泌体收集的总光谱的0.4-1.2%，而MFGM蛋白质组收集的总光谱占15-28%。这些数据表明，乳外泌体分泌途径与MFGM显著不同，部分原因是MFGM蛋白的存在大大减少。乳外泌体的蛋白质组成为乳蛋白组成和外泌体对乳腺生理的潜在生理意义提供了新的信息。Elsevier B.V.出版
Exosomes are 40-100 nm membrane vesicles of endocytic origin, secreted by cells and are found in biological fluids including milk. These exosomes are extracellular organelles important in intracellular communication, and immune function. Therefore, the proteome of bovine milk exosomes may provide insight into the complex processes of milk production. Exosomes were isolated from the milk of mid-lactation cows. Purified exosomes were trypsin digested, subjected offline high pH reverse phase chromatography and further fractionated on a nanoLC connected to tandem mass spectrometer. This resulted in identification of 2107 proteins that included all of the major exosome protein markers. The major milk fat globule membrane (MFGM) proteins (Butyrophilin, Xanthine oxidase, Adipophilin and Lactadherin) were the most abundant proteins found in milk exosomes. However, they represented only 0.4-1.2% of the total spectra collected from milk exosomes compared to 15-28% of the total spectra collected in the MFGM proteome. These data show that the milk exosome secretion pathway differs significantly from that of the MFGM in part due to the greatly reduced presence of MFGM proteins. The protein composition of milk exosomes provides new information on milk protein composition and the potential physiological significance of exosomes to mammary physiology. Published by Elsevier B.V.