Erythrocyte arginase activity as an indicator of lead exposure
Erythrocyte arginase activity as an indicator of lead exposure
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红细胞精氨酸酶活性作为铅暴露的指标
DOI：
10.1136/oem.40.1.106
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
S. Horiguchi
中科院分区：
文献类型：
--
作者：
K. Fukumoto;I. Karai;Y. Nishikawa;S. Horiguchi
摘要 开发了一种使用干血斑测定红细胞精氨酸酶活性的半自动化方法，该方法很容易在工厂现场制备以供以后的实验室分析。 49 名因职业接触铅的男性的红细胞平均精氨酸酶活性为 62·9 IU/g·Hb（SD，14·4 IU/g·Hb）；在 45 名未接触铅的男性中，平均值为 44·6 IU/g·Hb（SD，11·6 IU/g·Hb）。在接触铅的工人的样本中发现平均精氨酸酶活性明显较高（p < 0·001）。血铅与红细胞精氨酸酶之间的相关系数为r = 0·67 (p < 0·001)。血铅与精氨酸酶等铅指标的相关程度为：尿δ-氨基乙酰丙酸r = 0·75，红细胞精氨酸酶r = 0·67，尿铅r = 0·66，粪卟啉r = 0·63。红细胞精氨酸酶与肝功能检查、GOT、GPT、血清白蛋白无显着相关性。以40 μg/100 g血铅浓度为基础值，以56·2 IU/g·Hb红细胞精氨酸酶活性为铅作业人员筛查值时，精氨酸酶试验的敏感性和特异性分别为0·96和0·65。因此测试的效度计算为1·61。这些结果表明，红细胞精氨酸酶水平可以被认为是铅职业接触的重要指标之一，因为它很好地反映了铅在人体内的剂量反应关系。我们的方法可以快速分析红细胞精氨酸酶，因此应可用于筛查铅暴露。
ABSTRACT A semi-automated method has been developed for the determination of the arginase activity of erythrocytes using dried blood spots, which are easy to prepare on site in a factory for later laboratory analysis. The mean arginase activity of erythrocytes in 49 men occupationally exposed to lead was 62·9 IU/g·Hb (SD, 14·4 IU/g·Hb); in 45 men not exposed to lead the mean was 44·6 IU/g·Hb (SD, 11·6 IU/g·Hb). A significantly higher mean arginase activity was found in the specimens from lead-exposed workers (p < 0·001). The correlation coefficient between blood lead and erythrocyte arginase was r = 0·67 (p < 0·001). The degree of correlation between blood lead and lead indicators including arginase was r = 0·75 for urine δ-aminolaevulinic acid, r = 0·67 for erythrocyte arginase, r = 0·66 for urine lead, and r = 0·63 for coproporphyrin. Erythrocyte arginase showed no significant correlation in the liver function tests, GOT, GPT, and albumin in serum. When 40 μg/100 g of blood lead concentration was fixed as the basic value and 56·2 IU/g·Hb of erythrocyte arginase activity was set as the screening value in lead-exposed workers, the sensitivity and specificity of the arginase test were 0·96 and 0·65, respectively. Thus the validity of the test was calculated to be 1·61. These results show that the arginase level of erythrocytes can be considered to be one of the significant indicators of occupational exposure to lead because it reflects well the dose-response relationship of lead in the human body. Our method allows rapid analysis of erythrocyte arginase and thus should be useful in screening for lead exposure.
经肝膈肌超声诊断小儿心脏手术后膈神经麻痹的有用性：前瞻性观察研究，第一份报告
DOI：
--
发表时间：
2018
期刊：
影响因子：
--
作者：
田島聖士;飛田護邦;大下高志;水野博司;田畑雄一,橋本悟,山崎正記,井上美帆
通讯作者：
田畑雄一,橋本悟,山崎正記,井上美帆