Expression of NF-κB in prostate cancer lymph node metastases
Expression of NF-κB in prostate cancer lymph node metastases
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DOI：
10.1002/pros.10335
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发表时间：
2004-02-15
期刊：
影响因子：
2.8
通讯作者：
Saad, F
中科院分区：
文献类型：
--
作者：
Ismail, H;Lessard, L;Saad, F
导论.核因子-κ B（NF-κ B）是一种转录因子，其反式激活参与细胞生长、凋亡、血管生成和转移调节的基因。我们的目的是评估NF-kappaB在前列腺癌淋巴结转移中的表达。使用p65抗NF-κ B抗体进行免疫组织化学染色。分析了从54名前列腺癌患者中获得的77份石蜡包埋LN标本。在54例患者中，32例有淋巴结转移，22例无淋巴结转移，作为对照。评估NF-κ B核定位的总体百分比以及染色强度。与对照组相比，转移性LN组中NF-κ B的核定位显著更高。在LN阳性转移的患者中，84.4%的肿瘤细胞核染色>10%。此外，64.4%的恶性LN标本中淋巴细胞核染色>10%，而对照组为0%。淋巴结转移组细胞质和细胞核染色强度均高于对照组（P < 0.01）。NF-κ B的核定位/活化在前列腺癌LN转移中上调在肿瘤细胞以及周围淋巴细胞中观察到NF-κ B活性的这种上调。(C)2003 Wiley-Liss，Inc.
INTRODUCTION. Nuclear factor-kappaB (NF-kappaB) is a transcription factor that transactivates genes involved in the regulation of cell growth, apoptosis, angiogenesis, and metastasis. Our aim was to assess NF-kappaB expression in lymph node (LN) metastases of prostate cancer.METHODS. Immunohistochemical staining was performed using the p65 anti-NF-kappaB antibody. Seventy-seven paraffin-embedded LN specimens obtained from 54 prostate cancer patients were analyzed. Of the 54 patients, 32 had positive LN metastases, while 22 showed no evidence of metastasis and were considered as controls. The overall percentage of NF-kappaB-nuclear localization was assessed, as well as the intensity of staining.RESULTS. Nuclear localization of NF-kappaB was significantly greater in the metastatic LN group compared to controls. In patients with positive-LN metastases, 84.4% showed >10% nuclear staining in tumor cells. Moreover, 64.4% of the malignant LN specimens had >10% nuclear staining in lymphocytes compared to 0% in controls. Intensity of cytoplasmic and nuclear staining was higher in the metastatic LN group than in controls (P < 0.01).CONCLUSIONS. Nuclear localization/activation of NF-kappaB is up-regulated in prostate cancer LN metastasis. Such up-regulation of NF-kappaB activity is observed in the tumor cells as well as in the surrounding lymphocytes. (C) 2003 Wiley-Liss, Inc.