AN ENZYME-IMMUNOASSAY FOR THE DETECTION OF FLORIDA RED TIDE BREVETOXINS
AN ENZYME-IMMUNOASSAY FOR THE DETECTION OF FLORIDA RED TIDE BREVETOXINS
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DOI：
10.1016/0041-0101(91)90126-c
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发表时间：
1991-01-01
期刊：
影响因子：
2.8
通讯作者：
BADEN, DG
中科院分区：
文献类型：
--
作者：
TRAINER, VL;BADEN, DG
本文建立了一种非竞争性固相酶免疫分析法，用于检测不同基质中的短尾丝虫毒素。该测定利用山羊中产生的抗短毒素PbTx-3-钥孔血蓝蛋白缀合物的抗体，通过蛋白G和短毒素亲和柱特异性纯化短毒素抗体，以及与辣根过氧化物酶共价连接的兔抗山羊抗体。该试验专门用于检测细胞培养物和污染组织中的短尾孢毒素。测定的灵敏度为0.04皮摩尔，并且通过与标准曲线比较，可以在微量滴定板中从0.04 pM至0.4 pM短毒素/孔定量毒素。
A noncompetitive solid-phase enzyme immunoassay for detection of brevetoxins in various matrices has been developed. The assay utilizes antibodies raised in a goat against brevetoxin PbTx-3-keyhole limpet hemocyanin conjugates with specific purification of brevetoxin antibodies through protein G and brevetoxin affinity columns, and rabbit anti-goat antibodies covalently linked to horseradish peroxidase. The assay was used specifically to detect brevetoxins in both cell culture and contaminated tissues. Sensitivity of the assay is 0.04 picomolar, and toxin can be quantified from 0.04 pM to 0.4 pM brevetoxin per well in microtiter plates by comparison with standard curves.