Application of second harmonic imaging microscopy to assess structural changes in optic nerve head structure ex vivo
Application of second harmonic imaging microscopy to assess structural changes in optic nerve head structure ex vivo
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DOI：
10.1117/1.2717540
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Jester, James V.
中科院分区：
文献类型：
--
作者：
Brown, Donald J.;Morishige, Naoyuki;Jester, James V.
青光眼是全球第二大致盲原因。虽然年龄和眼压(IOP)都是公认的这种疾病的危险因素，但潜在的病理过程涉及视网膜神经节细胞(RGC)的加速死亡，这与进行性视力丧失有关。视神经节细胞的丢失主要是由于其解剖特征和筛板的机械脆弱性造成的视神经头(ONH)内轴突的损伤，筛板是由胶原束组成的专门的ONH区，定义了轴突束通过其离开眼睛的通道或毛孔。利用飞秒激光从胶原蛋白产生二次谐波(SH)信号的多光子显微技术的最新进展使筛板的直接光学成像成为可能。我们评估了SH生成显微镜(SHG)在ONH研究中的应用，并检验了ONH的一般假设，即同一只眼的眼压升高会导致ONH胶原束的移动，导致筛板通道的扭曲和轴突束的压缩。(C)2007年，光学仪器工程师学会。
Glaucoma represents the second leading cause of blindness worldwide. While both age and intraocular pressure (IOP) are wellrecognized risk factors for this disease, the underlying pathologic process involves the accelerated death of retinal ganglion cells (RGCs) that is associated with progressive loss of vision. The loss of RGCs has been postulated to occur primarily by injury to axons in the optic nerve head (ONH) due to its anatomic features and the mechanical vulnerability of the lamina cribrosa, the specialized ONH zone comprised of collagen beams that define the channels or pores through which axon bundles exit the eye. Recent advances in multiphoton microscopy using femtosecond lasers that generate second harmonic (SH) signals from collagen allows for direct optical imaging of the lamina cribrosa. We assess the application of SH generated microscopy (SHG) to the study of the ONH, and test the general hypothesis that increasing intraocular pressure in the same eye results in the movement of ONH collagen beams leading to distortion of the lamina cribrosa channels and compression of the axon bundles. (C) 2007 Society of Photo-Optical Instrumentation Engineers.