Exploring Pathogenic Mechanisms of Botrytis cinerea Secretome under Different Ambient pH Based on Comparative Proteomic Analysis
Exploring Pathogenic Mechanisms of Botrytis cinerea Secretome under Different Ambient pH Based on Comparative Proteomic Analysis
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基于比较蛋白质组学分析探讨不同环境pH下灰葡萄孢分泌组的致病机制
DOI：
10.1021/pr300365f
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发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Tian, Shiping
中科院分区：
文献类型：
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作者：
Li, Boqiang;Wang, Weihao;Tian, Shiping
灰霉病每年在全球200多种植物上引起灰霉病，造成巨大的经济损失。灰霉病菌分泌的蛋白质之间的协同作用对其成功侵染寄主植物起着重要作用。环境pH作为最重要的环境参数之一，可以调节各种真菌病原菌分泌蛋白的表达。本研究采用基于双向凝胶电泳的比较蛋白质组学方法，主要研究了环境pH对灰霉病菌B05.10菌株分泌组的影响。利用MALDI-TOF/TOF技术对灰霉病菌分泌组进行了鉴定，共鉴定出47个差异点，分别对应于21个独特的蛋白质。在pH为4时，诱导了更多与蛋白降解相关的蛋白质，而在pH为6时，向上积累的蛋白质大多是细胞壁降解酶。实时荧光定量PCR对基因表达的分析表明，这些蛋白质的产生大多是在转录水平上调节的。这些结果表明，灰霉菌能够调节分泌体的蛋白质谱以响应不同的环境pH值，为深入了解灰霉菌对多种植物寄主的复杂侵染机制提供了证据。
Botrytis cinerea causes gray mold rot on over 200 plant species worldwide, resulting in great economic loss every year. Cooperation of proteins secreted by B. cinerea plays an important role in its successful infection to host plants. The ambient pH, as one of the most important environmental parameters, can regulate expression of secreted proteins in various fungal pathogens. In the present study, we mainly investigated the effect of ambient pH on secretome of B. cinerea strain B05.10 with a comparative proteomic method based on 2-DE. Distinct differences in secretome of B. cinerea were found between pH 4 and 6 treatments, and 47 differential spots, corresponding to 21 unique proteins, were identified using MALDI-TOF/TOF. At pH 4, more proteins related to proteolysis were induced, whereas most of up-accumulated proteins were cell wall degrading enzymes at pH 6. Analysis of gene expression using quantitative real-time PCR suggests that production of most of these proteins was regulated at the level of transcription. These findings indicate that B. cinerea can adjust protein profile of secretome responding to different ambient pH values and provide evidence to deeply understand the complicated infecting mechanisms of B. cinerea on a wide range of plant hosts.