Multilayer Membranes of Glycosaminoglycans and Collagen I Biomaterials Modulate the Function and Microvesicle Release of Endothelial Progenitor Cells
Multilayer Membranes of Glycosaminoglycans and Collagen I Biomaterials Modulate the Function and Microvesicle Release of Endothelial Progenitor Cells
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糖胺聚糖和 I 型胶原蛋白生物材料的多层膜调节内皮祖细胞的功能和微泡释放
DOI：
10.1155/2016/4796578
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发表时间：
2016-01-01
影响因子：
4.3
通讯作者：
Ma, Xiaotang
中科院分区：
文献类型：
--
作者：
Dai, Bingyan;Pan, Qunwen;Ma, Xiaotang
生物材料的多层复合膜可以增强脂肪干细胞或骨祖细胞的功能。近年来的研究表明，内皮祖细胞（EPCs）及其释放的微囊泡（MV）在血管生成和血管修复中发挥重要作用。本文研究了透明质酸（HA）或硫酸软骨素（CS）和I型胶原（Col I）生物材料多层膜对EPCs功能和MVs释放的影响。采用层接层（LBL）技术构建多层复合膜。研究了四种类型的膜，通过吸附HA或CS和Col I交替与不同的顶层构建。结果表明，4种多层复合膜均能促进EPCs的增殖和迁移，抑制细胞衰老、凋亡和活化caspase-3的表达。有趣的是，这些生物材料增加了EPCs-MV的释放和miR-126水平。此外，顶层具有CS的CS-Col I膜显示出促进EPCs增殖、EPCs-MV释放和EPCs-MV中miR-126水平的最大效果。综上所述，HA/CS和Collagen I组成的多层复合膜可促进EPCs功能和富含miR-126的EPCs-MVs的释放，为组织修复治疗提供了一种新的策略。
Multilayer composite membrane of biomaterials can increase the function of adipose stem cells or osteoprogenitor cells. Recent evidence indicates endothelial progenitor cells (EPCs) and EPCs released microvesicles (MVs) play important roles in angiogenesis and vascular repair. Here, we investigated the effects of biomaterial multilayer membranes of hyaluronic acid (HA) or chondroitin sulfate (CS) and Collagen I (Col I) on the functions and MVs release of EPCs. Layer-by-layer (LBL) technology was applied to construct the multilayer composite membranes. Four types of the membranes constructed by adsorbing either HA or CS and Col I alternatively with different top layers were studied. The results showed that all four types of multilayer composite membranes could promote EPCs proliferation and migration and inhibit cell senility, apoptosis, and the expression of activated caspase-3. Interestingly, these biomaterials increased the release and the miR-126 level of EPCs-MVs. Moreover, the CS-Col I membrane with CS on the top layer showed the most effects on promoting EPCs proliferation, EPCs-MV release, and miR-126 level in EPCs-MVs. In conclusion, HA/CS and Collagen I composed multilayer composite membranes can promote EPCs functions and release of miR-126 riched EPCs-MVs, which provides a novel strategy for tissue repair treatment.