Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
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通过拉曼和表面增强拉曼光谱化学结合来区分单糖。
DOI：
10.1039/d2an01762h
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Schultz,ZacharyD
中科院分区：
文献类型：
--
作者：
Schorr,HannahC;Schultz,ZacharyD
糖在许多生物过程中发挥着重要作用，从提供能量到修饰蛋白质以改变其功能。糖基化，即糖残基与蛋白质的连接，是最常见的翻译后修饰。鉴定蛋白质上的聚糖是药物开发以及进一步探测蛋白质组和糖组的有用工具。然而，糖由于其异构性质而难以探测。在这项工作中，拉曼光谱和表面增强拉曼光谱（SERS）被用来识别不同的单糖物种的基础上，这些异构体分析物的振动模式。糖的弱散射通过与苯基硼酸缀合来克服，以提供更大的拉曼散射截面，并引起与单糖结构相关的观察到的光谱的轻微变化。进行自发拉曼、流动中的SERS和静态SERS检测以区分阿拉伯糖、果糖、半乳糖、葡萄糖、甘露糖和核糖，以及提供用于鉴定和定量这些糖缀合物的方法。
Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function. Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification. Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further. Sugars, however, are difficult analytes to probe due to their isomeric nature. In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes. The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides. Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.