Aggregatibacter actinomycetemcomitans serotype f O-polysaccharide mediates coaggregation with Fusobacterium nucleatum.
Aggregatibacter actinomycetemcomitans serotype f O-polysaccharide mediates coaggregation with Fusobacterium nucleatum.
复制标题
O-多糖血清型放线聚集杆菌介导与具核梭杆菌的共聚集。
DOI：
10.1111/j.1399-302x.2007.00399.x
复制
发表时间：
2008
影响因子：
--
通讯作者：
Kaplan,JB
中科院分区：
文献类型：
--
作者：
Rupani,D;Izano,EA;Schreiner,HC;Fine,DH;Kaplan,JB
背景/目的：细菌属间的共聚集可能在菌斑的形成中起重要作用。方法：在本研究中，我们研究了两种牙周致病菌Aggregatibacteractinomycetemcomitans和Fusobacteriumnucleatum之间的共聚集反应。以往的研究表明，放线放线菌伴放线菌血清型B菌株与核梭菌菌株PK 1594共聚集，放线菌共生血清型B O-多糖（O-PS）是引起伴放线放线菌和具核梭菌之间共聚集的受体已证明f型O-PS在结构和抗原性上与血清型B O-PS相关。 在本研究中，我们发现，放线放线菌共生菌株CU 1060 N，一个血清型f菌株，也共聚集与F. nucleatum PK 1594。 与B型菌株和具核梭菌之间的共聚集相似，半乳糖抑制了CU 1060 N和具核梭菌之间的共聚集。 CU 1060 N的O-PS突变体不能与具核梭菌共聚集。结论：放线共生放线放线杆菌血清型f O-PS与B O-PS一样，也能介导放线共生放线杆菌与梭菌的共聚集。
Background/aims:Intergeneric bacterial coaggregation may play an important role in plaque development.Methods:In this study we investigated the coaggregation reaction between two periodontal pathogens,Aggregatibacter actinomycetemcomitansandFusobacterium nucleatum.Results:Previous studies showed thatA. actinomycetemcomitansserotype b strains coaggregate withF. nucleatumstrain PK1594, and thatA. actinomycetemcomitansserotype b O‐polysaccharide (O‐PS) is the receptor responsible for coaggregation betweenA. actinomycetemcomitansandF. nucleatum.A. actinomycetemcomitansserotype f O‐PS has been shown to be structurally and antigenically related to serotype b O‐PS. In the present study we show thatA. actinomycetemcomitansstrain CU1060N, a serotype f strain, also coaggregated withF. nucleatumPK1594. Like coaggregation between serotype b strains andF. nucleatum, coaggregation between CU1060N andF. nucleatumwas inhibited by galactose. An O‐PS mutant of CU1060N failed to coaggregate withF. nucleatum.Conclusion:We concluded thatA. actinomycetemcomitansserotype f O‐PS, like serotype b O‐PS, mediates coaggregation betweenA. actinomycetemcomitansand fusobacteria.