Immunohistochemical evidence for 11β-hydroxylase (P45011β) and androgen production in the gonad during sex differentiation and in adults in the protandrous anemonefish Amphiprion clarkii
Immunohistochemical evidence for 11β-hydroxylase (P45011β) and androgen production in the gonad during sex differentiation and in adults in the protandrous anemonefish Amphiprion clarkii
复制标题
DOI：
10.2108/zsj.25.212
复制
发表时间：
2008-02-01
影响因子：
0.9
通讯作者：
Nakamura, Masaru
中科院分区：
文献类型：
--
作者：
Miura, Saori;Horiguchi, Ryo;Nakamura, Masaru
为了获得基本信息的内分泌机制的基础性变化的Protandrous Anemonefish的Amphiprion clarkii，我们研究了类固醇生成酶细胞色素11 β-羟化酶（P45011 β），这是参与11-酮睾酮（11-KT）的生产，免疫定位，并分析了性腺的能力，在整个性别分化过程中产生类固醇激素，并在繁殖阶段。对P45011 β的免疫阳性反应出现在性未分化的性腺在孵化后30天（dph）。免疫阳性细胞的数量继续增加，在卵巢分化（从60至180 dph），并在整个形成两性性腺与卵巢和睾丸组织，直到270 dph。在雄性阶段，在睾丸和卵巢组织的细胞间隙中观察到强免疫阳性细胞。P45011 β仅定位于卵泡膜细胞中，在雌性阶段包围发育的卵母细胞。在体外11-KT生产的性腺逐渐增加睾丸分化（前，分化期间和分化后）。雄性期性腺中11-KT的产生量高于睾丸分化期和雌性期。我们的研究结果表明，雄激素参与睾丸分化的性分化和精子发生。
To obtain basic information on the endocrine mechanisms underlying sex change in the protandrous anemonefish Amphiprion clarkii, we examined the immunolocalization of the steroidogenic enzyme cytochrome 11 beta-hydroxylase (P45011 beta), which is involved in 11-ketotestosterone (11-KT) production, and analyzed the ability of gonads to produce steroid hormones throughout the sex differentiation process and at the breeding stage. Immunopositive reactions against P45011 beta appeared in sexually undifferentiated gonads at 30 days post hatching (dph). The number of immunopositive cells continued to increase during ovarian differentiation (from 60 to 180 dph) and throughout the formation of ambisexual gonads with both ovarian and testicular tissue until 270 dph. In the male phase, strongly immunopositive cells were observed in the cellular interstices of both testicular and ovarian tissues. P45011 beta was localized only in the theca cells enclosing developed oocytes in the female phase. In-vitro 11-KT production in the gonads gradually increased with testicular differentiation (before, during, and after differentiation). Production of 11-KT in the gonads was higher in the male phase than during testicular differentiation or in the female phase. Our results suggest that androgen is involved in testicular differentiation during sex differentiation and spermatogenesis.